지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 279 - 285 (7page)
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초록· 키워드
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Background:Extended-spectrum cephalosporins (ESCs)-resistant Enterobacter cloacae is one of the pathogens of nosocomial infection the incidence of which is on the rise. Moreover, plasmid-mediated quinolone resistance genes (PMQR genes) that reduce quinolone sensitivity have been shown to be widely distributed among clinical isolates of Enterobacteriaceae. This study was carried out to observe the distribution of PMQR genes in clinical isolates of E. cloacae resistant to ESCs.
Materials and Methods:Fourty-three ESCs-resistant E. cloacae strains from the blood collected during the span of 7 years, from 1994 to 2001, at Seoul National University Children’s Hospital (SNUCH) were included in this study. Isoelectric focusing and enzyme specific PCR were performed to characterize β-lactamase. The presence of qnrA, qnrB, qnrC, qnrS, qepA, and aac(6’)-Ib-cr was determined by PCR, restriction enzyme analyses of PCR products, and DNA sequencing. Minimal inhibitory concentration (MIC) of several quinolones was measured by agar dilution method.
Results:The PMQR genes were detected in 9 (21%) of 43 ESCs-resistant E. cloacae isolates. Among them, five isolates were positive for qnrB2, and each two isolates harbored qnrB4 or qnrB5, respectively. qnrA, qnrC, qnrS or qepA was not identified. aac(6’)-Ib was detected in 27 isolates, but aac(6’)-Ib-cr was not found. Among the 9 qnrB-positive isolates, 5 produced SHV-12, 3 were derepressed mutants, and 1 produced pI 7.5 β-lactamase. MIC ranges and percent resistances of nalidixic acid, ofloxacin, levofloxacin, and moxifloxacin for the PMQR genes-positive isolates were higher than PMQR genes-negative isolates.
Conclusions:In this study, ESCs-resistant E. cloacae showed a high prevalence of PMQR genes, and qnrB was the only PMQR gene identified.
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참고문헌 (22)
참고문헌 신청
Cavaco LM / 2009 / qnrD, a novel gene conferring transferable quinolone resistance in Salmonella enterica serovar Kentucky and Bovismorbificans strains of human origin / Ant
Clinical and Laboratory Standards Institute (CLSI; formerly National committee for Clinical Laboratory Standard) / 2003 / Methods for Dilution Antimicrobial Susceptibility Tests for Bact
Garau J / 1999 / Emergence and dissemination of quinolone-resistant Escherichia coli in the community / Antimicrob Agents Chemother 43 : 2736 ~ 2741
Graffunder EM / 2005 / Risk factors associated with extended?spectrum beta?lactamase?producing organisms at a tertiary care hospital / J Antimicrob Chemother 56 : 139 ~ 145
Hata M / 2005 / Cloning of a novel gene for quinolone resistance from a transferable plasmid in Shigella flexneri 2b / Antimicrob Agents Chemother 49 : 801 ~ 803
Clinical and Laboratory Standards Institute (CLSI; formerly National committee for Clinical Laboratory Standard) / 2003 / Methods for Dilution Antimicrobial Susceptibility Tests for Bact
Garau J / 1999 / Emergence and dissemination of quinolone-resistant Escherichia coli in the community / Antimicrob Agents Chemother 43 : 2736 ~ 2741
Graffunder EM / 2005 / Risk factors associated with extended?spectrum beta?lactamase?producing organisms at a tertiary care hospital / J Antimicrob Chemother 56 : 139 ~ 145
Hata M / 2005 / Cloning of a novel gene for quinolone resistance from a transferable plasmid in Shigella flexneri 2b / Antimicrob Agents Chemother 49 : 801 ~ 803
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