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- 2010.1
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Human fibroblasts that maintain the structural integrity of connective tissues by secreting precursors of the extracellular matrix are typically cultured with serum.
However, there are potential disadvantages of the use of serum including unnatural interactions between the cells and the potential for exposure to animal pathogens. To prevent the possible influence of serum on fibroblast cultures, we devised a serum-free growth method and present in vitro data that demonstrate its suitability for growing porcine fetal fibroblasts. These cells were grown under four different culture conditions: no serum (negative control), 10% fetal bovine serum (FBS, positive control),10% knockout serum replacement (KSR) and 20% KSR in the medium. The proliferation rates and viabilities of the cells were investigated by counting the number of cells and trypan blue staining, respectively. The 10% FBS group showed the largest increase in the total number of cells (1.09 × 105cells/ml). In terms of the rate of viable cells, the results from the KSR supplementation groups (20% KSR:64.7%; 10% KSR: 80.6%) were similar to those from the 10% FBS group (68.5%). Moreover, supplementation with either 10% (3.0 × 104cells/ml) or 20% KSR (4.8 × 104cells/ml) produced similar cell growth rates. In conclusion,although KSR supplementation produces a lower cell proliferation rate than FBS, this growth condition is more effective for obtaining an appropriate number of viable porcine fetal fibroblasts in culture. Using KSR in fibroblast culture medium is thus a viable alternative to FBS.
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참고문헌 (18)
참고문헌 신청
Amit M / 2004 / Feeder layer- and serum-free culture of human embryonic stem cells / Biol of Reprod 70 : 837 ~ 845
Bettiol E / 2007 / Fetal bovine serum enables cardiac differentiation of human embryonic stem cells / Differentiation 75 : 669 ~ 681
Byers BA / 2008 / Transient exposure to transforming growth factor beta 3 under serumfree conditions enhances the biomechanical and biochemical maturation of tissue-engineere
Cheng J / 2004 / Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media / Genesis 39 : 100 ~ 104
Contard P / 1993 / Collagen fibrillogenesis in a three-dimensional fibroblast cell culture system / Cell Tissue Res 273 : 571 ~ 575
Bettiol E / 2007 / Fetal bovine serum enables cardiac differentiation of human embryonic stem cells / Differentiation 75 : 669 ~ 681
Byers BA / 2008 / Transient exposure to transforming growth factor beta 3 under serumfree conditions enhances the biomechanical and biochemical maturation of tissue-engineere
Cheng J / 2004 / Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media / Genesis 39 : 100 ~ 104
Contard P / 1993 / Collagen fibrillogenesis in a three-dimensional fibroblast cell culture system / Cell Tissue Res 273 : 571 ~ 575
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