지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 5 - 12 (8page)
이용수
초록· 키워드
오류제보하기
Recent studies indicate that reactive oxygen species (ROS)can act as modulators of neuronal activity, and are critically involved in persistent pain primarily through spinal mechanisms.
In this study, we investigated the effects of NaOCl, a ROS donor, on neuronal excitability and the intracellular calcium concentration ([Ca2+]i) in spinal substantia gelatinosa (SG)neurons. In current clamp conditions, the application of NaOCl caused a membrane depolarization, which was inhibited by pretreatment with phenyl-N-tert-buthylnitrone (PBN), a ROS scavenger. The NaOCl-induced depolarization was not blocked however by pretreatment with dithiothreitol, a sulfhydrylreducing agent. Confocal scanning laser microscopy was used to confirm whether NaOCl increases the intracellular ROS level. ROS-induced fluorescence intensity was found to be increased during perfusion of NaOCl after the loading of 2′,7′-dichlorofluorescin diacetate (H2DCF-DA). NaOCl-induced depolarization was not blocked by pretreatment with external Ca2+ free solution or by the addition of nifedifine.
However, when slices were pretreated with the Ca2+ ATPase inhibitor thapsigargin, NaOCl failed to induce membrane depolarization. In a calcium imaging technique using the Ca2+-sensitive fluorescence dye fura-2, the [Ca2+]i was found to be increased by NaOCl. These results indicate that NaOCl activates the excitability of SG neurons via the modulation of the intracellular calcium concentration, and suggest that ROS induces nociception through a central sensitization.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Avshalumov MV / 2002 / NMDA receptor activation mediates hydrogen peroxide-induced pathophysiology in rat hippocampal slices / J Neurophysiol 87 : 2896 ~ 2903
Bao L / 2005 / Partial mitochondria inhibition causes striatal dopamine release suppression and medium spiny neuron depolarization via H2O2 elevation, not ATP depletion / J
Baran CP / 2004 / The role of ROS and RNA in regulating life and death of blood monocytes / Curr Pharm 10 : 855 ~ 866
Bessac BF / 2008 / TRPA1 is a major oxidant sensor in murine airway sensory neurons / J Clin Invest 118 (5) : 1899 ~ 1910
Bubici C / 2006 / The NF-kappaB-mediated control of ROS and JNK signaling / Histol Histopathol 21 (1) : 69 ~ 80
Bao L / 2005 / Partial mitochondria inhibition causes striatal dopamine release suppression and medium spiny neuron depolarization via H2O2 elevation, not ATP depletion / J
Baran CP / 2004 / The role of ROS and RNA in regulating life and death of blood monocytes / Curr Pharm 10 : 855 ~ 866
Bessac BF / 2008 / TRPA1 is a major oxidant sensor in murine airway sensory neurons / J Clin Invest 118 (5) : 1899 ~ 1910
Bubici C / 2006 / The NF-kappaB-mediated control of ROS and JNK signaling / Histol Histopathol 21 (1) : 69 ~ 80
이 논문의 저자 정보
최근 본 자료
댓글(0)
0