지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
DNA found at the scene of a crime is often damaged and degraded to small fragments and remained in low quantity. Therefore, there are some difficulties in forensic STR typing of these DNA samples. In this study, to overcome these limitations as different approach, we applied repairing enzyme to damaged DNA. The efficacy of a repair enzyme system (PreCR Repair Mix) was evaluated by using several types of artificially damaged and naturally damaged DNA. The results showed that autosomal STR amplification produced increased yield in the DNA damaged by UV irradiation,oxidation, or acid/heat, and DNA from dried blood spot and dried saliva spot by treatment of a repair enzyme, but not in DNA extracted from old skeletal remains. In conclusion,a repair enzyme will be efficiently applied to forensic samples which were damaged by UV irradiation, oxidation, and acid/heat.
목차
등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Baron CP / 2006 / Oxidation of bovine serum albumin initiated by the Fenton reaction-effect of EDTA, tert-butylhydroperoxide and tetrahydrofuran / Free Radic Res 40 : 409 ~
Butler JM / 2003 / The development of reduced size STR amplicons as tools for analysis of degraded DNA / J Forensic Sci 48 : 1054 ~ 1064
Butler JM / 2005 / Forensic DNA typing. Biology, technology and genetics of STR markers. 2nd ed / Elsevier Academic Press
Diegoli TM / / An optimized protocol for forensic application of the PreCRTM Repair Mix to multiplex STR amplification of UV-damaged DNA / Forensic Sci Int Genet
Gill P / 2000 / An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA / Forensic Sci Int 112 : 17 ~ 40
Butler JM / 2003 / The development of reduced size STR amplicons as tools for analysis of degraded DNA / J Forensic Sci 48 : 1054 ~ 1064
Butler JM / 2005 / Forensic DNA typing. Biology, technology and genetics of STR markers. 2nd ed / Elsevier Academic Press
Diegoli TM / / An optimized protocol for forensic application of the PreCRTM Repair Mix to multiplex STR amplification of UV-damaged DNA / Forensic Sci Int Genet
Gill P / 2000 / An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA / Forensic Sci Int 112 : 17 ~ 40
이 논문의 저자 정보
최근 본 자료
댓글(0)
0