지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한소아알레르기호흡기학회 Allergy Asthma & Respiratory Diseases Allergy Asthma & Respiratory Diseases 제2권 제4호
- 발행연도
- 2014.1
- 수록면
- 259 - 265 (7page)
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초록· 키워드
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Purpose: Cow’s milk protein is one of the most common and strongest food allergen. We investigated the effects of heat treatment on the distribution and antigenicities of major allergens from cow’s milk. We also compared the protein distribution and antigenicities among cow’s milk formula and its substitutes.
Methods: We heated α-casen, β-lactoglobulin (BLG), α-lactalbumin (ALA), and crude extract of cow’s milk in 100°C boiling water for 1 hour. We prepared crude extracts from cow’s milk formula, partially hydrolyzed milk formula (pHF) and extensively hydrolyzed milk formula (eHF). The protein compositions of all the samples were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The antigenicities were determined by IgE immunoblotting with pooled serum collected from 11 patients with milk allergy.
Results: After heating, no significant alteration was found in casein, and the aggregates of ALA and BLG were detected with molecular weights of about 30 and 45 kDa, respectively. The antigenicities of newly detected aggregates were increased. The new aggregates of BLG with increased antigenicities were also found in heated milk total protein. Major milk allergens were not found in pHF, and residual components with a molecular weight below 10 KDa did not show IgE-binding activity. We failed to observe the residual components and antigenicities of eHF.
Conclusion: Changes in protein distribution and antigenicity of milk total protein induced by heat treatment may not be significantly different from those of each major allergen. The residual components of pHF could have little IgE-binding capacity, and there may be few or no antigenic components in eHF.
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참고문헌 (25)
참고문헌 신청
Bu G / 2013 / Milk processing as a tool to reduce cow's milk allergenicity: a mini-review / Dairy Sci Technol 93 : 211 ~ 223
Chung CS / 2012 / FDA's health claim review: whey-protein partially hydrolyzed infant formula and atopic dermatitis / Pediatrics 130 : e408 ~ e414
Davis PJ / 2001 / How can thermal processing modify the antigenicity of proteins? / Allergy 56 (67) : 56 ~ 60
Docena GH / 1996 / Iden
Gjesing B / 1986 / Allergen-specific IgE antibodies against antigenic components in cow milk and milk substitutes / Allergy 41 : 51 ~ 56
Chung CS / 2012 / FDA's health claim review: whey-protein partially hydrolyzed infant formula and atopic dermatitis / Pediatrics 130 : e408 ~ e414
Davis PJ / 2001 / How can thermal processing modify the antigenicity of proteins? / Allergy 56 (67) : 56 ~ 60
Docena GH / 1996 / Iden
Gjesing B / 1986 / Allergen-specific IgE antibodies against antigenic components in cow milk and milk substitutes / Allergy 41 : 51 ~ 56
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