지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 124 - 130 (7page)
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Purpose Current chemotherapeutics for treating locally advanced or metastatic soft tissue sarcomas (STS) are limited. Accordingly, the present in vitro study was conducted to evaluate the effects of treatment of STS cells with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) applied as a single agent or in combination with a proteasome inhibitor, MG132.
Materials and Methods Sensitivity to TRAIL and activity of TRAIL-induced apoptotic pathways were analyzed in four STS cell lines: HTB-82 (rhabdomyosarcoma), HT-1080 (fibrosarcoma), HTB-93 (synovial sarcoma), and HTB-94 (chondrosarcoma). Reduction of the dye dimethylthiazolyl 2,5diphenyltetrazolium bromide (MTT) was used to evaluate cytotoxic activity; western blots were used to evaluate TRAIL-induced apoptosis.
Results TRAIL induced apoptosis in HTB-93 cells, but had little effect in HTB-82, HT-1080, or HTB-94cells. Expression of TRAIL receptor-1 and -2 did not correlate with sensitivity to TRAIL. Coincubation of cells with TRAIL and a proteasome inhibitor, MG132, augmented the apoptotic effect of TRAIL in both TRAIL-sensitive and TRAIL-resistant cells. This effect was due to upregulation of TRAIL receptors and members of the pro-apoptotic BCL-2 family by MG132.
Conclusion These data show that combining TRAIL with MG132 enhances apoptosis and overcomes TRAIL resistance. This restoration of TRAIL sensitivity occurs through an increase in the expression of death receptor 5 and of pro-apoptotic BCL-2 family members such as BAX.
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참고문헌 (22)
참고문헌 신청
Adams J / 2004 / The proteasome: a suitable antineoplastic target / Nat Rev Cancer 4 : 349 ~ 360
Antman K / 1993 / An intergroup phase III randomized study of doxorubicin and dacarbazine with or without ifosfamide and mesna in advanced soft tissue and bone sarcomas / J C
Ashkenazi A / 2002 / Targeting death and decoy receptors of the tumour-necrosis factor superfamily / Nat Rev Cancer 2 : 420 ~ 430
Chen JJ / 2008 / Proteasome inhibitors enhance TRAILinduced apoptosis through the intronic regulation of DR5: involvement of NF-kappa B and reactive oxygen species-mediated
Clarkson P / 2004 / Management of soft tissue sarcomas of the extremities / Expert Rev Anticancer Ther 4 : 237 ~ 246
Antman K / 1993 / An intergroup phase III randomized study of doxorubicin and dacarbazine with or without ifosfamide and mesna in advanced soft tissue and bone sarcomas / J C
Ashkenazi A / 2002 / Targeting death and decoy receptors of the tumour-necrosis factor superfamily / Nat Rev Cancer 2 : 420 ~ 430
Chen JJ / 2008 / Proteasome inhibitors enhance TRAILinduced apoptosis through the intronic regulation of DR5: involvement of NF-kappa B and reactive oxygen species-mediated
Clarkson P / 2004 / Management of soft tissue sarcomas of the extremities / Expert Rev Anticancer Ther 4 : 237 ~ 246
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