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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2003.1
- 수록면
- 489 - 496 (8page)
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초록· 키워드
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Purpose: Small cell lung cancer is one of the major causes
of death from cancer worldwide. To explore the expressions
of global protein in small cell lung cancer cells, a
proteomic approach, to identify the proteins, was used and
the establishment of a protein reference map attempted.
Materials and Methods: Two-dimensional gel electrophoresis
(2-DE), with subsequent analysis by mass
spectrometry (MS), was applied to the study of protein
identification from a small cell lung cancer cell line, NCIH211.
The cells were lysed, and the extracts subjectedto
isoelectric focusing, with immobilized pH gradients, followed
by second dimension SDS-PAGE. The polypeptides
were identified by peptide mass fingerprinting, with
MALDI-TOF MS, after in-gel protein digestion.
Results: From silver staining of the gel, around two
thousands protein spots were separated by the 2-DE. Of
these protein spots visualized in the gel, one hundred and
ten were identified by peptide mass fingerprinting.
Different proteins, such as enzymes, cytoskeletal proteins
and proteins common to eukaryotic cells, were identified.
Conclusion: The protein expressions of the small cell
lung cancer cells were analyzed to establish a protein
reference map. The reference map presented here may
serve as a working tool for the further study of small cell
lung cancer. (Cancer Res Treat. 2003;35:489-496)
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