지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 338 - 349 (12page)
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Purpose Adipose stromal cells (ASCs) play an important regulatory role in cancer progression and metastasis by regulating systemic inflammation and tissue metabolism. This study examined whether visceral and subcutaneous ASCs (V- and S-ASCs) facilitate the growth and migration of ovarian cancer cells.
Materials and Methods CD45– and CD31– double-negative ASCs were isolated from the subcutaneous and visceral fat using magnetic-activated cell sorting. Ovarian cancer cells were cultured in conditioned media (CM) obtained from ASCs to determine the cancer-promoting effects of ASCs. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, Boyden chamber assay, and western blotting were performed to determine the proliferative activity, migration ability, and activation of the JAK2/STAT3 pathway, respectively.
Results CM from ASCs enhanced the migration of the ovarian cancer line, SKOV3, via activation of the JAK2/STAT3 signaling pathway. Interestingly, in response to ASC-CM, the ascites cells derived from an ovarian cancer patient showed an increase in growth and migration. The migration of ovarian cancer cells was suppressed by blocking the activation of JAK2 and STAT3 using a neutralizing antibody against interleukin 6, small molecular inhibitors (e.g., WP1066 and TG101348), and silencing of STAT3 using siRNA. Anatomical differences between S- and V-ASCs did not affect the growth and migration of the ovarian cancer cell line and ascites cells from the ovarian cancer patients.
Conclusion ASCs may regulate the progression of ovarian cancer, and possibly provide a potential target for anticancer therapy.
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참고문헌 (24)
참고문헌 신청
Baglioni S / 2012 / Functional differences in visceral and subcutaneous fat pads originate from differences in the adipose stem cell / PLoS One 7 : e36569 ~
Bjorntorp P / 1996 / The regulation of adipose tissue distribution in humans / Int J Obes Relat Metab Disord 20 : 291 ~ 302
Coffelt SB / 2009 / The pro-inflammatory peptide LL-37promotes ovarian tumor progression through recruitment of multipotent mesenchymal stromal cells / Proc Natl Acad Sci U
Gilbert CA / 2013 / Cytokines, obesity, and cancer : new insights on mechanisms linking obesity to cancer risk and progression / Annu Rev Med 64 : 45 ~ 57
Ibrahim MM / 2010 / Subcutaneous and visceral adipose tissue : structural and functional differences / Obes Rev 11 : 11 ~ 18
Bjorntorp P / 1996 / The regulation of adipose tissue distribution in humans / Int J Obes Relat Metab Disord 20 : 291 ~ 302
Coffelt SB / 2009 / The pro-inflammatory peptide LL-37promotes ovarian tumor progression through recruitment of multipotent mesenchymal stromal cells / Proc Natl Acad Sci U
Gilbert CA / 2013 / Cytokines, obesity, and cancer : new insights on mechanisms linking obesity to cancer risk and progression / Annu Rev Med 64 : 45 ~ 57
Ibrahim MM / 2010 / Subcutaneous and visceral adipose tissue : structural and functional differences / Obes Rev 11 : 11 ~ 18
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