지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The purpose of this study is to investigate the influence of organism vigor information solution(OVIS)on the animal cell growth and to set up a condition for cell culture. We investigated the reactions on the MG-63and MCF-7 cell line in mixture culture media with various amount of REVIEW solution, which is an example among various OVIS. DMEM-HG was used as basic media. The concentration range of the mixture was limited from 0%to 15%. MTT assay is used for cell viability test. The cell was incubated for 14days and the MTT assay was performed on day 1, 3, 7, 10 and 14 throughout the experiment. We used the ELISA reader to measure the Optical Density(O.D) at 595 nm wavelength filter. MCF-7 was linearly proliferated according to culture time and concentrations of OVIS. On the other hand, MG-63 cells were measured the highest O.D value at 12%. The growth rates of both cells cultured in mixed culture media with OVIS are much higher than those in only basic media, DMEMHG,after 14days. It was confirmed that cell cultured at OVIS grows rapidly at certain period although cells showed a negative effect in initial stage.
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참고문헌 (25)
참고문헌 신청
/ / http://www.re-view.net/index.php
A. Billiau / 1977 / Human interferon: mass production in a newly established cell line, MG-63 / Antimicrob. Agents Chemother 12 : 11 ~ 15
A. Mobasheri / 2009 / Mesenchymal stem cells in connective tissue engineering and regenerative medicine: applications in cartilage repair and osteoarthritis therapy / Histol
A.Carrel / 1912 / Pure cultures of cells / J of Experimental Medicine 16 (2) : 165 ~ 168
A.G. Bodnar / 1998 / Extension of life-span by introduction of telomerase into normal human cells / Scienc 279 : 349 ~ 352
A. Mobasheri / 2009 / Mesenchymal stem cells in connective tissue engineering and regenerative medicine: applications in cartilage repair and osteoarthritis therapy / Histol
A.Carrel / 1912 / Pure cultures of cells / J of Experimental Medicine 16 (2) : 165 ~ 168
A.G. Bodnar / 1998 / Extension of life-span by introduction of telomerase into normal human cells / Scienc 279 : 349 ~ 352
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