지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이 논문의 연구 히스토리 (3)
2016
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
Tumor cell morphology is closely related to its migratory behaviors. An active tumor cell has a highly irregular shape, whereas a spherical cell is inactive. Thus, quantitative analysis of cell features is crucial to determine tumor malignancy or to test the efficacy of anticancer treatment. We use 3D time-lapse phase-contrast microscopy to analyze single cell morphology because it enables to observe long-term activity of living cells without photobleaching and phototoxicity,which is common in other fluorescence-labeled microscopy. Despite this advantage, there are image-level drawbacks to phase-contrast microscopy, such as local light effect and contrast interference ring. Therefore, we first corrected for non-uniform illumination artifacts and then we use intensity distribution information to detect cell boundary.
In phase contrast microscopy image, cell is normally appeared as dark region surrounded by bright halo ring. Due to halo artifact is minimal around the cell body and has non-symmetric diffusion pattern, we calculate cross sectional plane which intersects center of each cell and orthogonal to first principal axis. Then, we extract dark cell region by analyzing intensity profile curve considering local bright peak as halo area. Finally, we calculated the Fourier descriptor that morphological characteristics of cell to classify tumor cells into active and inactive groups. We validated classification accuracy by comparing our findings with manually obtained results.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
C. Decaestecker / 2007 / Can anti-migratory drugs be screened in vitro? A review of 2D and 3D assays for the quantitative analysis of cell migration / Med Res Rev 27 (2) : 1
C.-C. Chang / / LIBSVM: a library for support vector machines
F. Zernike / 1942 / Physica : 686 ~
H. Lee / 2010 / Histogram based Correction of Non-uniform Illumination on Phase-Contrast Microscopy / in Proc. Workshop on Image Processing and Image Understanding (IPIU) : 347 ~
I. Adanja / 2010 / Automated tracking of unmarked cells migrating in three-dimensional matrices applied to anti-cancer drug screening / Expl. Cell Res 316 : 181 ~ 193
C.-C. Chang / / LIBSVM: a library for support vector machines
F. Zernike / 1942 / Physica : 686 ~
H. Lee / 2010 / Histogram based Correction of Non-uniform Illumination on Phase-Contrast Microscopy / in Proc. Workshop on Image Processing and Image Understanding (IPIU) : 347 ~
I. Adanja / 2010 / Automated tracking of unmarked cells migrating in three-dimensional matrices applied to anti-cancer drug screening / Expl. Cell Res 316 : 181 ~ 193
이 논문의 저자 정보
최근 본 자료
댓글(0)
0