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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 69 - 75 (7page)
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초록· 키워드
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Background: By conventional methods, the identification of anaerobic bacteria is more time consuming and requires more expertise than the identification of aerobic bacteria. Although the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems are relatively less studied, they have been reported to be a promising method for the identification of anaerobes. We evaluated the performance of the VITEK MS in vitro diagnostic (IVD; 1.1 database; bioMérieux, France) in the identification of anaerobes.
Methods: We used 274 anaerobic bacteria isolated from various clinical specimens. The results for the identification of the bacteria by VITEK MS were compared to those obtained by phenotypic methods and 16S rRNA gene sequencing.
Results: Among the 249 isolates included in the IVD database, the VITEK MS correctly identified 209 (83.9%) isolates to the species level and an additional 18 (7.2%) at the genus level. In particular, the VITEK MS correctly identified clinically relevant and frequently isolated anaerobic bacteria to the species level. The remaining 22 isolates (8.8%) were either not identified or misidentified. The VITEK MS could not identify the 25 isolates absent from the IVD database to the species level.
Conclusions: The VITEK MS showed reliable identifications for clinically relevant anaerobic bacteria.
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등록된 정보가 없습니다.
참고문헌 (16)
참고문헌 신청
Chun J / 2007 / EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences / Int J Syst Evol Microbiol 57 : 2259 ~ 2261
Clark AE / 2013 / Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology / Clin M
Clinical and Laboratory Standards Institute / 2008 / Interpretive criteria for identification of bacteria and fungi by DNA target sequencing. Approved guideline, MM18-A / Clinical and
Culebras E / 2012 / Rapid identification of clinical isolates of Bacteroides species by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry / Anaerob
Dubois D / 2012 / Performances of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid identification of bacteria in ro
Clark AE / 2013 / Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology / Clin M
Clinical and Laboratory Standards Institute / 2008 / Interpretive criteria for identification of bacteria and fungi by DNA target sequencing. Approved guideline, MM18-A / Clinical and
Culebras E / 2012 / Rapid identification of clinical isolates of Bacteroides species by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry / Anaerob
Dubois D / 2012 / Performances of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid identification of bacteria in ro
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