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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 142 - 152 (11page)
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Background : In April 2009, a novel influenza A (H1N1) virus was detected in the US, and at the time of conducting this study, H1N1 infection had reached pandemic proportions. In Korea, rapid antigen tests and PCR assays have been developed to detect the H1N1 virus. We evaluated the efficacies of rapid antigen test, multiplex PCR, and real-time PCR for detecting the H1N1 virus.
Methods : From August to September 2009, we tested 734 samples obtained from nasopharyngeal swab or nasal swab using rapid antigen test (SD Influenza Antigen, Standard Diagnostics, Inc., Korea) and multiplex PCR (Seeplex FluA ACE Subtyping, Seegene, Korea). We also tested 224 samples using the AdvanSure real-time PCR (LG Life Sciences, Korea) to compare the results obtained using real-time PCR with those obtained using multiplex PCR. Furthermore, 99 samples were tested using the AdvanSure real-time PCR and the AccuPower real-time PCR (Bioneer, Korea).
Results : In comparison with the results of multiplex PCR, the sensitivity and specificity of the rapid antigen test were 48.0% and 99.8%, respectively. The concordance rate for multiplex PCR and the AdvanSure real-time PCR was 99.6% (k=0.991, P=0.000), and that for the AdvanSure real-time PCR and the AccuPower real-time PCR was 97.0% (k=0.936, P=0.000).
Conclusions : The rapid antigen test is significantly less sensitive than PCR assay; therefore, it is not useful for H1N1 detection; however multiplex PCR, the AdvanSure real-time PCR, and the Accu- Power real-time PCR can be useful for H1N1 detection. (Korean J Lab Med 2010;30:147-52)
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참고문헌 (16)
참고문헌 신청
Carr MJ / 2009 / Development of a real-time RT-PCR for the detection of swine-lineage influenza A (H1N1) virus infections / J Clin Virol 45 : 196 ~ 199
Centers for Disease Control and Prevention / 2009 / Evaluation of rapid influenza diagnostic tests for detection of novel influenza A (H1N1) Virus-United States, 2009 / MMWR
Ge Y / 2010 / Detection of novel swine origin influenza A virus (H1N1) by real-time nucleic acid sequencebased amplification / J Virol Methods 163 : 495 ~ 497
Ginocchio CC / 2009 / Evaluation of multiple test methods for the detection of the novel 2009 influenza A (H1N1) during the New York City outbreak / J Clin Virol 45 : 191 ~ 1
He J / 2009 / Rapid multiplex reverse transcription-PCR typing of influenza A and B virus, and subtyping of influenza A virus into H1, 2, 3, 5, 7, 9, N1 (human), N1 (animal)
Centers for Disease Control and Prevention / 2009 / Evaluation of rapid influenza diagnostic tests for detection of novel influenza A (H1N1) Virus-United States, 2009 / MMWR
Ge Y / 2010 / Detection of novel swine origin influenza A virus (H1N1) by real-time nucleic acid sequencebased amplification / J Virol Methods 163 : 495 ~ 497
Ginocchio CC / 2009 / Evaluation of multiple test methods for the detection of the novel 2009 influenza A (H1N1) during the New York City outbreak / J Clin Virol 45 : 191 ~ 1
He J / 2009 / Rapid multiplex reverse transcription-PCR typing of influenza A and B virus, and subtyping of influenza A virus into H1, 2, 3, 5, 7, 9, N1 (human), N1 (animal)
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