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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치과보철학회 The Journal of Advanced of Prosthodontics The Journal of Advanced of Prosthodontics 제7권 제1호
- 발행연도
- 2015.1
- 수록면
- 21 - 26 (6page)
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초록· 키워드
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PURPOSE. To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs). MATERIALS AND METHODS. Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco’s modified eagle’s culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments. RESULTS. All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P<.001). When the cells were exposed to media by Ultratemp, the cell viability was similar to that of the control at 24 hours (P>.05); however, the cell viability was significantly reduced at 72 hours (P<.001). Light and scanning electron microscopy examination confirmed these results. CONCLUSION. The cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations.
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참고문헌 (30)
참고문헌 신청
Sunzel B / 1997 / The protective effect of zinc on rosin and resin acid toxicity in human polymorphonuclear leukocytes and human gingival fibroblasts in vitro / J Biomed Mater Res 37:20~28
Hume WR / 1994 / Influence of dentine on the pulpward release of eugenol or acids from restorative materials / J Oral Rehabil 21:469~473
Hanks CT / 1994 / Permeability of biological and synthetic molecules through dentine / J Oral Rehabil 21:475~487
Schmalz G / 2000 / Influence of albumin and collagen on the cell mortality evoked by zinc oxide-eugenol in vitro / J Endod 26:284~287
/ 2009 / ISO 10993-5. Biological evaluation of medical devices - Part 5:Tests for in vitro cytotoxicity / International Standards Organization (ISO)
Hume WR / 1994 / Influence of dentine on the pulpward release of eugenol or acids from restorative materials / J Oral Rehabil 21:469~473
Hanks CT / 1994 / Permeability of biological and synthetic molecules through dentine / J Oral Rehabil 21:475~487
Schmalz G / 2000 / Influence of albumin and collagen on the cell mortality evoked by zinc oxide-eugenol in vitro / J Endod 26:284~287
/ 2009 / ISO 10993-5. Biological evaluation of medical devices - Part 5:Tests for in vitro cytotoxicity / International Standards Organization (ISO)
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