지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 120 - 125 (6page)
이용수
초록· 키워드
오류제보하기
목적: 본 연구는 in-vitro 상에서 recombinant human transforming growth factor-beta (rhTGF-β2)와 poly(D,L-lactide-co-glycolide) (PLGA) 의 복합체를 티타늄 디스크 표면에 코팅하여, 생물학적으로 간엽줄기세포 증식에 미치는 영향을 조사하기 위해 시행되었다.
재료 및 방법: 양극산화 디스크에 일렉트로스프레이 코팅법을 이용하여 anodized 된 티타늄 디스크를 대조군으로 설정하고, rhTGF-β2를 125 ng/ml와 500 ng/ml 농도로 코팅한 것을 실험군으로 하였다. 티타늄 디스크 표면에 분사된 복합체가 균일하게 분사되었는지 field-emission scanning electron microscopy (FE-SEM)을 통해 확인하였으며, atomic force microscope (AFM) test를 이용하여 rhTGF-β2로 코팅한 디스크와 양극산화 디스크의 거칠기 차이를 확인하였다. 디스크 위에 간엽줄기세포 배양 후 1, 4, 7일에 세포증식 양상을 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl-tetrazolium bromide) assay 검사를 통해 확인하였다.
결과: AFM 결과 대조군과 실험군에서 거칠기의 유의할만한 차이가 없었다 (P>.05). MTT 결과 7일차 배양 결과에서 125 ng/ml와 500 ng/ml PLGA/TGF-β2처리된 그룹은 각각 평균 0.45와 0.48이였으며, 대조군은 평균 0.33으로 PLGA/TGF-β2처리된 그룹에서 세포 증식이 더 활성화 되는 것을 확인할 수 있었다 (P<.05).
결론: rhTGF-β2 복합체를 electrospray법으로 코팅한 티타늄 표면에서 7일차에서 줄기간엽세포의 빠른 증식을 확인하였다. 또한 복합체 처리군의 농도가 증가할수록 높은 세포 성장 수치를 보였다.
목차
등록된 정보가 없습니다.
참고문헌 (29)
참고문헌 신청
Hermann JS / 1997 / Crestal bone changes around titanium implants. A radiographic evaluation of unloaded nonsubmerged and submerged implants in the canine mandible / J Periodontol 68:1117~1130
Sul YT / 2002 / Characteristics of the surface oxides on turned and electrochemically oxidized pure titanium implants up to dielectric breakdown: the oxide thickness, micropore configurations, surface roughness, crystal structure and chemical composition / Biomaterials 23:491~501
Xie J / 2004 / Osteoblasts respond to hydroxyapatite surfaces with immediate changes in gene expression / J Biomed Mater Res A 71:108~117
Ivanoff CJ / 2001 / Histologic evaluation of the bone integration of TiO(2)blasted and turned titanium microimplants in humans / Clin Oral Implants Res 12:128~134
Orsini G / 2000 / Surface analysis of machined versus sandblasted and acid-etched titanium implants / Int J Oral Maxillofac Implants 15:779~784
Sul YT / 2002 / Characteristics of the surface oxides on turned and electrochemically oxidized pure titanium implants up to dielectric breakdown: the oxide thickness, micropore configurations, surface roughness, crystal structure and chemical composition / Biomaterials 23:491~501
Xie J / 2004 / Osteoblasts respond to hydroxyapatite surfaces with immediate changes in gene expression / J Biomed Mater Res A 71:108~117
Ivanoff CJ / 2001 / Histologic evaluation of the bone integration of TiO(2)blasted and turned titanium microimplants in humans / Clin Oral Implants Res 12:128~134
Orsini G / 2000 / Surface analysis of machined versus sandblasted and acid-etched titanium implants / Int J Oral Maxillofac Implants 15:779~784
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