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논문 기본 정보

자료유형
학술저널
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대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제42권 제3호
발행연도
2012.1
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73 - 80 (8page)

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이 논문의 연구 히스토리 (2)

2017

Influences of immunosuppressants on the osteogenic activity of rat mesenchymal stem cells in vitro & in vivo
변유경 치의학과 2017.01 학위논문

2012

Effects of immunosuppressants, FK506 and cyclosporin A, on the osteogenic differentiation of rat mesenchymal stem cells
변유경 ,  김경화 ,  김수환 외 7명 Journal of Periodontal & Implant Science 2012.01 학술저널

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Purpose: The purpose of this study was to investigate the effects of the immunosuppressants FK506 and cyclosporin A (CsA)on the osteogenic differentiation of rat mesenchymal stem cells (MSCs). Methods: The effect of FK506 and CsA on rat MSCs was assessed in vitro. The MTT assay was used to determine the deleterious effect of immunosuppressants on stem cell proliferation at 1, 3, and 7 days. Alkaline phosphatase (ALP) activity was analyzed on days 3, 7, and 14. Alizarin red S staining was done on day 21 to check mineralization nodule formation. Real-time polymerase chain reaction (RT-PCR) was also performed to detect the expressions of bone tissue-specific genes on days 1 and 7. Results: Cell proliferation was promoted more in the FK506 groups than the control or CsA groups on days 3 and 7. The FK506groups showed increased ALP activity compared to the other groups during the experimental period. The ALP activity of the CsA groups did not differ from the control group in any of the assessments. Mineralization nodule formation was most prominent in the FK506 groups at 21 days. RT-PCR results of the FK506 groups showed that several bone-related genes-osteopontin,osteonectin, and type I collagen (Col-I)-were expressed more than the control in the beginning, but the intensity of expression decreased over time. Runx2 and Dlx5 gene expression were up-regulated on day 7. The effects of 50 nM CsA on osteonectin and Col-I were similar to those of the FK506 groups, but in the 500 nM CsA group, most of the genes were less expressed compared to the control. Conclusions: These results suggest that FK506 enhances the osteoblastic differentiation of rat MSCs. Therefore, FK506 might have a beneficial effect on bone regeneration when immunosuppressants are needed in xenogenic or allogenic stem cell transplantation to treat bone defects.
#Cell differentiation #Cyclosporin A #FK506 #Immunosuppressive agents #Mesenchymal stem cells.

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참고문헌 (23)

참고문헌 신청
Caplan AI / 2007 / Adult mesenchymal stem cells for tissue engineering versus regenerative medicine / J Cell Physiol 213 : 341 ~ 347 google schola Cvetkovic M / 1994 / The deleterious effects of long-term cyclosporine A, cyclosporine G, and FK506 on bone mineral metabolism in vivo / Transplantation 57 : 1231 ~ 1237 google schola Epstein S / 1996 / Post-transplantation bone disease: the role of immunosuppressive agents and the skeleton / J Bone Miner Res 11 : 1 ~ 7 google schola Fukunaga J / 2004 / Expression of osteoclast differentiation factor and osteoclastogenesis inhibitory factor in rat osteoporosis induced by immunosuppressant FK506 / Bone 34 google schola Isomoto S / 2007 / Rapamycin as an inhibitor of osteogenic differentiation in bone marrow-derived mesenchymal stem cells / J Orthop Sci 12 : 83 ~ 88 google schola

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