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학술저널
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대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제34권 제2호
발행연도
2004.1
수록면
357 - 366 (10page)

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Chronic exposure to high levels of manganese leads a pronounce and debilitating disorder known as manganism. Research on the toxic manifestation of manganese have focused primarily on its neurological effects because exposure to high levels of the metal produces a distinct and irreversible extrapyramidal dysfunction resembling the dystonic movements associated with Parkinson's physiological and biochemical systems in the body. The purpose of this study was to evaluate the effect of manganeses on primary rat calvarial cell growth and toxicity. The experimental groups were in concentration of 0, 10, 30, 60, 100, 300 μ M. Cell activity was assessed at day 1 and day 3 using a fluorescent molecular probe. Cell proliferation was evaluated at day 1 and day 3 by MTT assay. The amount of total protein synthesis was measured at day 3 and day 7. The results were as follows: The proliferation of primary rat calvarial cells were inhibited by MnCl2 in the concentration exceeding 100μM. The primary rat calvarial cells treated with MnCl2 showed similar protein synthesis to the control group except in 100 μM. These result suggest that manganese suppress the viability and protein synthesis of primary rat calvarial cells in concentration exceeding 100μM.

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