5-Aminolevulinic Acid(ALA)를 이용한 광역학 치료:in vitro, in vivo에서의 항암효과에 관한 연구

오충훈; 김영생; 박지연; 정필상; 이상준; 정상용; 김지선

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자료유형: 학술저널

저자정보: 오충훈 김영생 박지연 정필상 이상준 정상용 김지선

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제48권 제2호

발행연도: 2005.1

수록면: 234 - 240 (7page)

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연구주제

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연구배경

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연구방법

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연구결과

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Background and Objectives：ALA is a precursor of heme and converted to protoporphyrin IX used as effective photosensitizer. The aim of this study was to find the ideal concentration and incubation time of ALA for PDT on in vitro and in vivo experiments and to assess the anti-tumor effect of PDT using ALA on CT-26 colon cancer cell line. Materials and Method：CT-26 cell was cultured with serum free media including ALA in the dark room to show the intracellular accumulation of PpIX. The fluorescence of PpIX in the cell was detected under confocal laser scanning microscope. Also CT-26 cell was incubated with various concentration of ALA (1.0-0.001 mg/ml) and was irradiated with LED at 0 hr, 3 hr, 6 hr, 9 hr, 12 hr and 24 hr after application of ALA. The cell viability was assessed by MTT assay. In vivo PDT was done with optimal treatment condition and the anti-tumor effect of PDT using ALA was measured by tumor volume change. Results：The fluorescence of PpIX was saturated at 6 hour after the ALA application to CT-26 cell and the optimal incubation time with ALA for PDT was 6 hours. For in vivo Study, 632 nm laser irradiation was done around the tumor 6 hours after ALA injection. The PDT using ALA on transplanted CT-26 tumors shows 40% cure rate and 40% partial remission and significant decrease of tumor volume. Conclusion：The peak accumulation of PpIX in the cell and in the tumor was reached 6 hours after the application of ALA. The PDT using ALA for CT-26 cells was very effective and this findings suggest that ALA is one of candidate for photosensitizer in head and neck solid tumors. (Korean J Otolaryngol 2005;48:234-40)

#ALA·광역학치료·광감작제. #Aminolevulinic acid· Photodynamic therapy· Photosensitizer.

참고문헌 (15)

참고문헌 신청

/ 1992 / Endogenous protoporphyrin X,a clinicalusefull photosensitizer for photodynamic therapy 14 : 275 ~ 92

/ 1994 / A mechanistic study ofcellularphotodestruction with 5-aminolevulinic acid induced porphyrin 70 : 21 ~ 8

/ 1994 / In vivo fluorescencekinetics and photodynamic therapy using 5-aminolevulinic acid inducedporphyrin:Increased damage after multiple irradiations 70 : 867 ~ 72

/ 1995 / Enhancement of photodynamic therapy with 5-aminolevulinicacid induced porphyrin photosensitization in normal rat colon bythreshold and light fractionation studies 7

/ 1995 / Photosensitisation and photodynamic therapy ofesophageal,duodenal,and colorectal tumors using 5-aminolevulinicacid induced protoporphyrin IX-a pilot study 36 : 67 ~

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