지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 277 - 286 (10page)
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초록· 키워드
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Purpose: To investigate the molecular responses of various genes and proteins relatedto disc degeneration upon treatment with cytokines that affect disc-cell proliferationand phenotype in living human intervertebral discs (IVDs). Responsivenessto these cytokines according to the degree of disc degeneration was also evaluated. Materials and Methods: The disc specimens were classified into two groups: group 1 (6 patients) showed mild degeneration of IVDs and group 2 (6 patients)exhibited severe degeneration of IVDs. Gene expression was analyzed after treatment with four cytokines: recombinant human bone morphogenic protein (rhBMP-2), transforming growth factor-β (TGF-β), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α). Molecular responses were assessed after exposure of cells from the IVD specimens to these cytokines via real-time polymerase chain reaction and immunofluorescence staining. Results: mRNA gene expression was significantly greater for aggrecan, type I collagen, type II collagen, alkaline phosphatase,osteocalcin, and Sox9 in group 1 than mRNA gene expression in group 2, when the samples were not treated with cytokines. Analysis of mRNA levels for these molecules after morphogen treatment revealed significant increases in both groups, which were much higher in group 1 than in group 2. The average number of IVD cells that were immunofluorescence stained positive for alkaline phosphataseincreased after treatment with rhBMP-2 and TGF-β in group 1. Conclusion: The biologic responsiveness to treatment of rhBMP-2, TGF-β, TNF-α, and IL-1β in the degenerative living human IVD can be different according to the degree of degenerationof the IVD.
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참고문헌 (21)
참고문헌 신청
Alini M / 2002 / A biological approach to treating disc degeneration: not for today, but maybe for tomorrow / Eur Spine J 11 (Suppl 2) : S215 ~ s220
An HS / 2005 / Intradiscal administration of osteogenic protein-1 increases intervertebral disc height and proteoglycan content in the nucleus pulposus in normal adolescent r
Antoniou J / 1996 / The human lumbar intervertebral disc: evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, agein
Antoniou J / 2004 / Apparent diffusion coefficient of intervertebral discs related to matrix composition and integrity / Magn Reson Imaging 22 : 963 ~ 972
Brisby H / 2004 / Cell therapy for disc degeneration--potentials and pitfalls / Orthop Clin North Am 35 : 85 ~ 93
An HS / 2005 / Intradiscal administration of osteogenic protein-1 increases intervertebral disc height and proteoglycan content in the nucleus pulposus in normal adolescent r
Antoniou J / 1996 / The human lumbar intervertebral disc: evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, agein
Antoniou J / 2004 / Apparent diffusion coefficient of intervertebral discs related to matrix composition and integrity / Magn Reson Imaging 22 : 963 ~ 972
Brisby H / 2004 / Cell therapy for disc degeneration--potentials and pitfalls / Orthop Clin North Am 35 : 85 ~ 93
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