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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 7 - 12 (6page)
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초록· 키워드
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Objectives: The objective of this study is to investigate the effects of Salviae Miltiorrhizae Radix hot aqueous extract on nitric oxide (NO) and prostaglandin E2 (PGE2) production and on 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical scavenging in macrophages.
Methods: Salviae Miltiorrhizae Radix (300 g) was heated at 100℃ with distilled water (2 L) for 4 hours. The extract was filtered and concentrated to 100 mL by using a rotary evaporator, was frozen at -80℃, and was then freeze-dried by using a freezing-drying system. The RAW 264.7 macrophage was subcultured by using 10-㎍/mL lipopolysaccharide (LPS). In order to evaluate cytotoxicity, we performed (3-(4,5-dimrthylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assays and measured the cell viability. The NO production was measured by using Griess assays, and the PGE2 production was measured by using enzyme immunoassays. The antioxidant activity, the 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical scavenging capability, was measured by using the DPPH method.
Results: Cell viability with the 1-, 5-, 25-, 125- and 625-㎍/mL Salviae Miltiorrhizae Radix hot aqueous extract was not significantly decreased compared to the cell viability without the extract. When 125 and 625 ㎍/mL of Salviae Miltiorrhizae Radix hot aqueous extract were used, nitric oxide (NO) production in LPS-stimulated RAW 264.7 macrophages was significantly inhibited compared to that in the control group. When 25, 125, and 625 ㎍/mL of Salviae Miltiorrhizae Radix hot aqueous extract were used, PGE2 production in LPS-stimulated RAW 264.7 macrophages was significantly inhibited compared to that in the control group. The 125- and 625-㎍/mL Salviae Miltiorrhizae Radix hot aqueous extracts had high DPPH free-radical scavenging capabilities in RAW 264.7 macrophages.
Conclusion: This study indicates that Salviae Miltiorrhizae Radix hot aqueous extract suppresses NO and PGE2 production and improves DPPH free-radical scavenging capability. Thus, it seems that Salviae Miltiorrhizae Radix hot aqueous extract may have an anti-inflammation effect and antioxidant activity.
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참고문헌 (24)
참고문헌 신청
Bhattacharyya A. / 2002 / Mitogen-activated protein kinases regulate Mycobacterium avium-induced tumor necrosis factor-alpha release from macrophages / FEMS Immunol Med Micr
Blois MS. / 1958 / Antioxidant determination by the use of stable free radical / Nature 181 : 1199 ~ 1200
Bunn HF. / 1996 / Oxygen sensing and molecular adaptation to hypoxia / Physiol Rev 76 (3) : 839 ~ 885
Choi SM. / 2000 / Study on Antitumor and Apoptosis-Inducing Effects of Salviae miltiorrhizae Radix / Korean J Oriental Medical Pathology 14 (2) : 22 ~ 47
Helmer KS. / 2002 / Induction of NF-kappaB, IkappaB-alpha, and iNOS in rat gastric mucosa during endotoxemia / J Surg Res 104 (1) : 46 ~ 52
Blois MS. / 1958 / Antioxidant determination by the use of stable free radical / Nature 181 : 1199 ~ 1200
Bunn HF. / 1996 / Oxygen sensing and molecular adaptation to hypoxia / Physiol Rev 76 (3) : 839 ~ 885
Choi SM. / 2000 / Study on Antitumor and Apoptosis-Inducing Effects of Salviae miltiorrhizae Radix / Korean J Oriental Medical Pathology 14 (2) : 22 ~ 47
Helmer KS. / 2002 / Induction of NF-kappaB, IkappaB-alpha, and iNOS in rat gastric mucosa during endotoxemia / J Surg Res 104 (1) : 46 ~ 52
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