지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 32 - 41 (10page)
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Objectives: Rehmannia glutinosa pharmacopuncture solution (RGPS) was investigated to determine both its anti-allergic inflammatory effects on mast cells and its detailed mechanism of actions.
Methods: We investigated whether RGPS suppress cytokines, enzymes, FcεRI expression and FcεRI–mediated signaling in RBL-2H3 cells stimulated with anti-DNP IgE/DNP-HSA. The suppressive effects of RGPS on the levels of cytokines such as IL-1β, IL-6 and GM-CSF were measured using emzyme-linked immunospecific assay (ELISA). The mRNA expression levels of cytokines, enzymes (HDC2, COX-1, COX-2 and 5LO) and FcεRI αβγ subunits were measured using reverse transcription polymerase chain reaction (RT-PCR) method. The activation of FcεRI-mediated signaling was examined using Western blot analyses.
Results: RGPS suppressed production of pro-inflammatory cytokines (IL-1β, IL-6, and GM-CSF) in stimulated RBL-2H3 cells significantly (p < 0.05). RGPS also suppressed mRNA expression of inflammatory enzymes (HDC2, COX-1, COX-2, 5LO). In addition, mRNA expression levels of FcεRIα, FcεRIβ and FcεRIγ were lowered by treatment with RGPS. Finally, RGPS prevented phosphrylation of Lyn, Syk, LAT, Gab2, PLCγ1/2, PI3K, Akt, cPLA2 and IκBα.
Conclusion: RGPS effectively suppresses mast cell activations such as degranulation and inflammatory response via down-regulation of the FcεRI-mediated signaling pathways in IgE/Ag-stimulated mast cells.
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참고문헌 (23)
참고문헌 신청
Alvarez-Errico D. / 2009 / Adapters in the organization of mast cell signaling / Immunol Rev 232 (1) : 195 ~ 217
Gilfillan AM. / 2006 / Integrated signalling pathways for mast-cell activation / Nat Rev Immunol 6 (3) : 218 ~ 230
Hirata. / 1999 / Expression of histidine decarboxylase messenger RNA and histamine N-methyltransferase messenger RNA in nasal allergy / Clin Exp Allergy 29 (1) : 76 ~ 83
Hu. ltner L. / 2000 / In activated mast cells, IL-1 upregulates the production of several Th2-related cytokines including IL-9 / J Immunol 164 (11) : 5556 ~ 5563
Kandere-Grzybowska K. / 2003 / IL-1 induces vesicular secretion of IL-6 without degranulation from human mast cells / J Immunol 171 (9) : 4830 ~ 4836
Gilfillan AM. / 2006 / Integrated signalling pathways for mast-cell activation / Nat Rev Immunol 6 (3) : 218 ~ 230
Hirata. / 1999 / Expression of histidine decarboxylase messenger RNA and histamine N-methyltransferase messenger RNA in nasal allergy / Clin Exp Allergy 29 (1) : 76 ~ 83
Hu. ltner L. / 2000 / In activated mast cells, IL-1 upregulates the production of several Th2-related cytokines including IL-9 / J Immunol 164 (11) : 5556 ~ 5563
Kandere-Grzybowska K. / 2003 / IL-1 induces vesicular secretion of IL-6 without degranulation from human mast cells / J Immunol 171 (9) : 4830 ~ 4836
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