지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 15 - 24 (10page)
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초록· 키워드
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Background: Type I allergy is involved in allergic asthma, allergic rhinitis, and atopic dermatitis which are accompanied by an acute and chronic allergic inflammatory responses.
Rehmannia glutinosa is a traditional medicine in the East Asian region. This study examined whether a Rehmannia Glutinosa pharmacopuncture solution (RGPS) had anti-allergic or anti-inflammatory effects in antigen-stimulated-RBL-2H3 cells.
Methods: We determined the effect of RGPS on cell viability using the 3-[4,5-dimethylthiazolyl]-2,5-diphenyltetrazolium bromide (MTT) assay. We also examined the effect of RGPS on the release of β-hexosaminidase and the secretion of IL-4 and TNF-α using ELISA. In addition, we evaluated the effect of RGPS on the mRNA expression of various cytokines; IL-2, IL-3, IL-4, IL-5, IL-13 and TNF-α using RT-PCR. Furthermore, we assessed the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor (NF)-κB using Western blotting after RGPS treatment.
Results: We found that RGPS (10-4 to 10-1 dilution) did not cause any cytotoxicity. We observed significant inhibition of β-hexosaminidase release and suppression of the protein secretion of IL-4 and TNF-α and mRNA expression of multiple cytokines in antigen-stimulated-RBL-2H3 cells after RGPS treatment. Additionally, RGPS suppressed not only the phosphorylation of MAPKs, but also the transcriptional activation of NF-κB in antigen-stimulated-RBL-2H3 cells.
Conclusions: These results suggest that RGPS inhibits degranulation and expression of cytokines including IL-4 and TNF-α via down-regulation of MAPKs and NF-κB activation in antigen-stimulated-RBL-2H3 cells. In conclusion, RGPS may have beneficial effects in the exerting anti-allergic or anti-inflammatory activities.
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참고문헌 (24)
참고문헌 신청
Cao, Z.L. / 1988 / Effects of radix Rehmanniae and processed radix Rehmanniae on some immune functions in mice / Zhong Yao Tong Bao 13 (10) : 22 ~ 24
Frossi, B. / 2007 / Selective activation of Fyn/PI3K and p38 MAPK regulates IL-4 production in BMMC under nontoxic stress condition / J Immunol 178 (4) : 2549 ~ 2555
Ghosh, S. / 1998 / NF-kappa B and Rel proteins: evolutionarily conserved mediators of immune responses / Annu Rev Immunol 16 : 225 ~ 260
Gilfillan, A.M. / 2006 / Integrated signalling pathways for mast-cell activation / Nat Rev Immunol 6 (3) : 218 ~ 230
Gordon, J.R. / 1990 / Mast cells as a source of multifunctional cytokines / Immunol Today 11 (12) : 458 ~ 464
Frossi, B. / 2007 / Selective activation of Fyn/PI3K and p38 MAPK regulates IL-4 production in BMMC under nontoxic stress condition / J Immunol 178 (4) : 2549 ~ 2555
Ghosh, S. / 1998 / NF-kappa B and Rel proteins: evolutionarily conserved mediators of immune responses / Annu Rev Immunol 16 : 225 ~ 260
Gilfillan, A.M. / 2006 / Integrated signalling pathways for mast-cell activation / Nat Rev Immunol 6 (3) : 218 ~ 230
Gordon, J.R. / 1990 / Mast cells as a source of multifunctional cytokines / Immunol Today 11 (12) : 458 ~ 464
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