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Objective: To assess the effect of the ethyl chloride spray on motor unit action potential (MUAP) morphology to the needling area who undergoes needle electromyography. Methods: Twenty examinees were enrolled to see the effect of the variability of the motor unit action potentials during needle electromyography (EMG). After needle insertion in biceps brachii muscle, examinees contracted the muscle and quantitative electromyography motor unit action potentials were analyzed. After being analyzed, the ethyl chloride was sprayed over the site of needle insertion and then quantitative electromyography was carried out again. Results: Quantitative electromyography showed that the amplitude and phase were not changed in all examinee after ethyl chloride spraying. However the duration was elongated with significant statistical difference. Conclusions: Our results suggest that ethyl chloride spraying before needle EMG study could change MUAP duration parameters. So, we must consider such changes in analyzing needle EMG result when using ethyl chloride spraying

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