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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 571 - 577 (7page)
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초록· 키워드
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Phospholipase D (PLD) catalyzes the hydrolysis of phosphatidylcholine to generate the lipid second messenger, phosphatidic acid. PLD is localized in most cellular organelles, where it is likely to play different roles in signal transduction. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes and autophagosomes. However, the factors defining its localization are less clear. In this study, we found that four hydrophobic residues present in the N-terminal HKD catalytic motif of PLD1, which is involved in intramolecular association, are responsible for vesicular localization. Site-directed mutagenesis of the residues dramatically disrupted vesicular localization of PLD1. Interestingly, the hydrophobic residues of PLD1are also involved in the interruption of its nuclear localization. Mutation of the residues increased the association of PLD1 with importin-β, which is known to mediate nuclear importation, and induced the localization of PLD1 from vesicles into the nucleus. Taken together, these data suggest that the ydrophobic amino acids involved in the interdomain association of PLD1 are required for vesicular localization and disturbance of its nuclear localization.
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참고문헌 (23)
참고문헌 신청
Ahn BH / 2003 / Transmodulation between phospholipase D and c-Src enhances cell proliferation / Mol Cell Biol 23 : 3103 ~ 3115
Brown FD / 1998 / Phospholipase D1 localises to secretory granules and lysosomes and is plasma-membrane translocated on cellular stimulation / Curr Biol 8 : 835 ~ 838
Colley WC / 1997 / Cloning and expression analysis of murine phospholipase D1 / Biochem J 326 (Pt 3) : 745 ~ 753
Colley WC / 1997 / Phospholipase D2, a distinct phospholipase D isoform with novel regulatory properties that provokes cytoskeletal reorganization / Curr Biol 7 : 191 ~ 201
Du G / 2003 / Regulation of phospholipase D1 subcellular cycling through coordination of multiple membrane association motifs / J Cell Biol 162 : 305 ~ 315
Brown FD / 1998 / Phospholipase D1 localises to secretory granules and lysosomes and is plasma-membrane translocated on cellular stimulation / Curr Biol 8 : 835 ~ 838
Colley WC / 1997 / Cloning and expression analysis of murine phospholipase D1 / Biochem J 326 (Pt 3) : 745 ~ 753
Colley WC / 1997 / Phospholipase D2, a distinct phospholipase D isoform with novel regulatory properties that provokes cytoskeletal reorganization / Curr Biol 7 : 191 ~ 201
Du G / 2003 / Regulation of phospholipase D1 subcellular cycling through coordination of multiple membrane association motifs / J Cell Biol 162 : 305 ~ 315
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