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- 2015.1
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Fucoidan has attracted attention as a potential drug because of its biological activities, which include osteogenesis. However, the molecular mechanisms involved in the osteogenic activity of fucoidan in human alveolar bone marrow-derived mesenchymal stem cells (hABM-MSCs) remain largely unknown. We investigated the action of fucoidan on osteoblast differentiation in hABMMSCs and its impact on signaling pathways. Its effect on proliferation was determined using the crystal violet staining assay. Osteoblast differentiation was evaluated based on alkaline phosphatase (ALP) activity and the mRNA expression of multiple osteoblast markers. Calcium accumulation was determined by Alizarin red S staining. We found that fucoidan induced hABMMSC proliferation. It also significantly increased ALP activity, calcium accumulation and the expression of osteoblast-specific genes, such as ALP, runt-related transcription factor 2, type I collagen-α 1 and osteocalcin. Moreover, fucoidan induced the expression of bone morphogenetic protein 2 (BMP2) and stimulated the activation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase by increasing phosphorylation. However, the effect of fucoidan on osteogenic differentiation was inhibited by specific inhibitors of ERK (PD98059) and JNK (SP600125) but not p38 (SB203580). Fucoidan enhanced BMP2 expression and Smad 1/5/8, ERK and JNK phosphorylation. Moreover, the effect of fucoidan on osteoblast differentiation was diminished by BMP2 knockdown. These results indicate that fucoidan induces osteoblast differentiation through BMP2–Smad 1/5/8 signaling by activating ERK and JNK, elucidating the molecular basis of the osteogenic effects of fucoidan in hABM-MSCs.
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참고문헌 (24)
참고문헌 신청
Angstwurm K / 1995 / Fucoidin, a polysaccharide inhibiting leukocyte rolling, attenuates inflammatory responses in experimental pneumococcal meningitis in rats / Neurosci Le
Bilan MI / 2002 / Structure of a fucoidan from the brown seaweed Fucus evanescens C.Ag / Carbohydr Res 337 : 719 ~ 730
Boo HJ / 2011 / Fucoidan from Undaria pinnatifida induces apoptosis in A549 human lung carcinoma cells / Phytother Res 25 : 1082 ~ 1086
Changotade SI / 2008 / Potential effects of a low-molecular-weight fucoidan extracted from brown algae o
Chen G / 2012 / TGF-beta and BMP signaling in osteoblast differentiation and bone formation / Int J Biol Sci 8 : 272 ~ 288
Bilan MI / 2002 / Structure of a fucoidan from the brown seaweed Fucus evanescens C.Ag / Carbohydr Res 337 : 719 ~ 730
Boo HJ / 2011 / Fucoidan from Undaria pinnatifida induces apoptosis in A549 human lung carcinoma cells / Phytother Res 25 : 1082 ~ 1086
Changotade SI / 2008 / Potential effects of a low-molecular-weight fucoidan extracted from brown algae o
Chen G / 2012 / TGF-beta and BMP signaling in osteoblast differentiation and bone formation / Int J Biol Sci 8 : 272 ~ 288
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