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Type I allergy is an immunoglobulin E (IgE)-medi-ated hypersensitivity disease inflicting more thanquarter of the world population. In order to identifyallergen sources, skin provocation test and IgEserology was performed using allergen extracts.Such process identifies allergen-containing sourcesbut cannot identify the disease-eliciting allergenicmolecules. Recently, microaray technology hasbeen developed for allergen-specific IgE detectionusing rolling circle amplification. This study wascarried out to evaluate protein chip technology forthe quantitative measurement and limits of sensitivityof multiple allergen-specific IgE by an immunofluores-cence assay. Significance of positive calibrators wastested using purified human IgE. Dermatophagoidespteronyssinus (Dp), egg white, milk, soybean, andwheat were used as allergens and human serumalbumin as negative control. Sensitivity and clinicalefficacy of protein chip were evaluated using allergyimmune serum for Dp. The fluorescent intensitiesfor purified human IgE as calibrator were wel core-lated with the concentrations of human IgE. Two-fold dilution of serum allowed an optimal reactionwith Dp (1 mg/ml) at which serum Dp-specific IgElevels by protein chip were compatible with those byUniCap. The sensitivity of protein chip in thisstudy was found at level of 1 IU/ml of IgE. Dp-spe-cific IgE levels by protein chip corelated wel withthose of UniCap by comparing 10 atopic dermati-tis. Aditional 18 sera were tested for above multipleantigens other than Dp and significant results wereobtained for many antigens as well as Dp. Theseresults indicated that spotting of heterogeneousprotein mixture on protein chip and the quantitativemeasurement of serum allergen-specific IgE levelsusing immunofluorescence assay can be success-fully applied in the clinical laboratory for the diagno-sis of allergy and could be applied to diagnosis ofautoimmune and infectious diseases

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