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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 478 - 486 (9page)
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Osteoblasts can synthesize the insulin-like growth factors
(IGFs) and the IGF-binding proteins (IGFBPs),
which may either enhance or attenuate IGF-stimulated
bone cell proliferation. Since estrogen induced osteoblastic
differentiation and proliferation through an estrogen-
responsive gene in target cells, we investigated
the effects of estrogen on IGFBP-6 expression in the
human osteoblastic-like cell line SaOS-2. Expressions
of IGFBP-6 protein and mRNA increased 2.8 and 2-fold,
respectively, in the presence of 17-β-estradiol (E2)
(0.01 to 1 μM) and estrogen receptor (ER) in SaOS-2
cells. On the other hand, E2 induced a 2-fold increase
in SaOS-2 cell proliferation. To identify genomic sequences
associated with estrogen responsiveness,
the 5'-promoter region (-44 to +118) of the IGFBP-6 gene
was cloned into a chloramphenicol acetyltransferase
(CAT) reporter vector. E2 induced a 3-fold increase in
CAT activity in SaOS-2 cells transiently transfected
with this construct. Identification of the estrogen-
responsive element (ERE) [5'-CCTTCA CCTG-3']
(-9 to +1) in this IGFBP-6 gene promoter region was confirmed
using electromobility shift assays and deletion
analysis. This functional ERE was important for
E2-induced trans-activation of the IGFBP-6 gene.
These results demonstrate that E2 exhibits a positive
effect on IGFBP-6 gene transcription through estrogen-
liganded ER binding to the functional ERE in the
IGFBP-6 gene promoter in SaOS-2 cells.
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참고문헌 (22)
참고문헌 신청
Arts J / 1997 / Differential expression of estrogen receptors alpha and beta mRNA during differentiation of human osteoblast SV-HFO cells / Endocrinology 138 : 5067 ~ 5070
Bach LA / 1994 / Recombinant human insulin-like growth factor (IGF)-binding protein-6 inhibits IGF-II-induced differentiation of L6A1 myoblasts / Endocrinology 135 : 2168 ~ 2
Baddoo M / 2003 / Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection / J Cell Biochem 89 : 1235 ~ 1249
Bienvenu G / 2004 / Insulin-like growth factor binding protein-6 transgenic mice: postnatal growth, brain development, and reproduction abnormalities / Endocrinology 145 : 24
Bodine PV / 1998 / Estrogen receptor-alpha is developmentally regulated during osteoblast differentiation and contributes to selective responsiveness of gene expression / End
Bach LA / 1994 / Recombinant human insulin-like growth factor (IGF)-binding protein-6 inhibits IGF-II-induced differentiation of L6A1 myoblasts / Endocrinology 135 : 2168 ~ 2
Baddoo M / 2003 / Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection / J Cell Biochem 89 : 1235 ~ 1249
Bienvenu G / 2004 / Insulin-like growth factor binding protein-6 transgenic mice: postnatal growth, brain development, and reproduction abnormalities / Endocrinology 145 : 24
Bodine PV / 1998 / Estrogen receptor-alpha is developmentally regulated during osteoblast differentiation and contributes to selective responsiveness of gene expression / End
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