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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 457 - 463 (7page)
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Embryonic stem cells (ESCs) can be propagated in vitro on feeder layers of mouse STO fibroblast cells. The STO cells secrete several cytokines that are essential for ESCs to maintain their undifferentiated state. In this study, we found significant growth inhibition of mouse ESCs (mESCs) cultured on STO cells infected with adenovirus containing a dominant-negative mutant form of IκB (rAd-dnIκB). This blockage of the NF-κB signal pathway in STO cells led to a significant decrease in [3H]thymidine incorporation and colony formation of mESCs. Expression profile of cytokines secreted from the STO cells revealed an increase in the bone morphogenetic protein4 (BMP4) transcript level in the STO cells infected with adenoviral vector encoding dominant negative IκB (rAd-dnIκB). These results suggested that the NF-κB signaling pathway represses expression of BMP4 in STO feeder cells. Conditioned medium from the rAd-dnIκB-infected STO cells also significantly reduced the colony size of mESCs. Addition of BMP4 prevented colony formation of mESCs cultured in the conditioned medium. Our finding suggested that an excess of BMP4 in the conditioned medium also inhibits proliferation of mESCs.
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참고문헌 (24)
참고문헌 신청
[학술
Bhardwaj G / 2001 / Sonic hedgehog induces the proliferation of primitive human hematopoietic cells via BMP regulation / Nat Immunol 2 : 172 ~ 178
Bhatia M / 1997 / Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture / J Exp Med 186 : 619 ~ 624
Bhatia M / 1999 / Bone morphogenetic proteins regulate the developmental program of human hematopoietic stem cells / J Exp Med 189 : 1139 ~ 1148
Brons IG / 2007 / Derivation of pluripotent epiblast stem cells from mammalian embryos / Nature 448 : 191 ~ 195
Bhardwaj G / 2001 / Sonic hedgehog induces the proliferation of primitive human hematopoietic cells via BMP regulation / Nat Immunol 2 : 172 ~ 178
Bhatia M / 1997 / Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture / J Exp Med 186 : 619 ~ 624
Bhatia M / 1999 / Bone morphogenetic proteins regulate the developmental program of human hematopoietic stem cells / J Exp Med 189 : 1139 ~ 1148
Brons IG / 2007 / Derivation of pluripotent epiblast stem cells from mammalian embryos / Nature 448 : 191 ~ 195
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