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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 101 - 110 (10page)
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초록· 키워드
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Previously, we reported that CD40-induced production of reactive oxygen species (ROS) by NADPH oxidase requires the TNF receptor-associated factor (TRAF) 3, as well as the activities of phosphatidylinositol 3-kinase (PI3K) and Rac1. Here we investigated the possible mechanisms of the production of ROS after CD40 ligation in B cells. We describe an alternative ROS production pathway that is triggered by CD40 ligation,involves 5-lipoxygenase (5-LO), and results in activation of p38 MAPK. Our studies in Raji human B lymphomas revealed that CD40-induced ROS production by 5-LO also requires the activities of PI3K and Rac1. In contrast to the NADPH oxidase pathway, however,TRAF molecules are not required for the CD40-induced ROS production by 5-LO. The association of CD40 with 5-LO is dependent on CD40 ligation in Raji B cells, and co-immunoprecipitation experiments using epitopetagged proteins transiently expressed in human embryonic kidney 293T cells revealed the role of the regulatory subunit of PI3K, p85, in this association. Collectively,these data suggest a separate pathway for the CD40-induced ROS production in B cells and demonstrate that this pathway requires 5-LO via direct association of p85 with both CD40 and 5-LO.
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참고문헌 (24)
참고문헌 신청
Aagaard-Tillery KM / 1996 / Phosphatidylinositol 3-kinase activation in normal human B lymphocytes / J Immunol 156 (4543)
Andjelic S / 2000 / Phosphatidylinositol 3-kinase and NF-κB/Rel are at the divergence of CD40-mediated proliferation and survival pathways / J Immunol 165 : 3860 ~ 3867
Aslan M / 2003 / Oxidants in receptor tyrosine kinase signal transduction pathways / Antioxid Redox Signal 5 : 781 ~ 788
Bae YS / 2000 / Platelet-derived growth factor-induced H2O2 production requires the activation of phosphatidylinositol 3-kinase / J Biol Chem 275 : 10527 ~ 10531
Bererich I / 1994 / Cross-linking CD40 on B cells rapidly activates nuclear factor-κB / J Immunol 153 : 4357 ~ 4366
Andjelic S / 2000 / Phosphatidylinositol 3-kinase and NF-κB/Rel are at the divergence of CD40-mediated proliferation and survival pathways / J Immunol 165 : 3860 ~ 3867
Aslan M / 2003 / Oxidants in receptor tyrosine kinase signal transduction pathways / Antioxid Redox Signal 5 : 781 ~ 788
Bae YS / 2000 / Platelet-derived growth factor-induced H2O2 production requires the activation of phosphatidylinositol 3-kinase / J Biol Chem 275 : 10527 ~ 10531
Bererich I / 1994 / Cross-linking CD40 on B cells rapidly activates nuclear factor-κB / J Immunol 153 : 4357 ~ 4366
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