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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제14권 제4호
- 발행연도
- 2004.1
- 수록면
- 665 - 672 (8page)
이용수
초록· 키워드
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D-Ribose is a five-carbon sugar used for thecommercial synthesis of riboflavin, antiviral agents, and flavorenhancers. Batch fermentations with transketolase-deficientB. subtilis JY1 were carried out to optimize the production ofD-ribose from xylose. The best results for the fermentationwere obtained with a temperature of 37oC and an initial pH of7.0. Among various sugars and sugar alcohols tested, glucoseand sucrose were found to be the most effective for both cellgrowth and D-ribose production. The addition of 15 g/l xyloseand 15 g/l glucose improved the fermentation performance,presumably due to the adequate supply of ATP in the xylosemetabolism from D-xylulose to D-xylulose-5-phosphate. Abatch culture in a 3.7-l jar fermentor with 14.9 g/l xylose and13.1 g/l glucose resulted in 10.1 g/l D-ribose concentrationwith a yield of 0.62 g D-ribose/g sugar consumed, and 0.25 g/l-h of productivity. Furthermore, the sugar utilization profile,indicating the simultaneous consumption of xylose and glucose,and respiratory parameters for the glucose and sucrose mediasuggested that the transketolase-deficient B. subtilis JY1 lostthe glucose-specific enzyme II of the phosphoenolpyruvatetransferase system.
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참고문헌 (27)
참고문헌 신청
Atkins / 1998 / Physical Chemistry : 14 ~ 44
Chandrakant, P / 2000 / Application of acompatible xylose isomerase in simultaneous bioconversionof glucose and xylose to ethanol 5 : 32 ~ 39
Choi / 2000 / Production of xylitol in cell recycle fermentations ofCandida tropicalis 22 : 1625 ~ 1628
De Wulf / 1996 / D-Glucose does not catabolite repress a transketolasedeficientD-ribose-producing Bacillus subtilis mutant strain 17 : 104 ~ 109
De Wulf / 1997 / Optimization of D-ribose production with a transketolaseaffectedBacillus subtilis mutant strain in glucose andgluconic acid-base media 83 : 25 ~ 30
Chandrakant, P / 2000 / Application of acompatible xylose isomerase in simultaneous bioconversionof glucose and xylose to ethanol 5 : 32 ~ 39
Choi / 2000 / Production of xylitol in cell recycle fermentations ofCandida tropicalis 22 : 1625 ~ 1628
De Wulf / 1996 / D-Glucose does not catabolite repress a transketolasedeficientD-ribose-producing Bacillus subtilis mutant strain 17 : 104 ~ 109
De Wulf / 1997 / Optimization of D-ribose production with a transketolaseaffectedBacillus subtilis mutant strain in glucose andgluconic acid-base media 83 : 25 ~ 30
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