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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제7호
- 발행연도
- 2006.1
- 수록면
- 1,139 - 1,143 (5page)
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초록· 키워드
오류제보하기
Various techniques and strategies have beendeveloped for the identification of intracellular metabolicconditions, and among them, isotope balance-based fluxanalysis with gas chromatography/mass spectrometry (GC/MS) has recently become popular. Even though isotopebalance-based flux analysis allows a more accurate estimationof intracellular fluxes, its aplication has ben restricted torelatively smal metabolic systems because of the limitednumber of measurable metabolites. In this paper, a strategyfor incorporating isotope balance-based flux data obtained fora small network into metabolic flux analysis was examined asa feasible alternative alowing more accurate quantification ofintracelular flux distribution in a large metabolic system. Toimpose GC/MS based data into a large metabolic network andobtain optimum flux distribution profile, data reconciliation308 intracelular reactions could be estimated from 29 GC/MS based fluxes with higher accuracy.
목차
등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
Azuyuki Shimizu / 2002 / A Review on Metabolic Pathway Analysis with Emphasis on Isotope Labeling Approach / Biotechnology and Bioprocess Engineering 7 (5) : 237 ~ 251
Bailey, J. E / 1991 / Towards a science of metabolic engineering / Science 252 : 1668 ~ 1674
Ben-Israel, A / 1974 / Generalized inverses: Theory and applications / John Wiley & Sons, New York
Christensen, B / 2000 / Metabolic network analysis of P. chrisogenum using 13C-labeled glucose / Biotechnol. Bioeng. 68 : 652 ~ 659
Fischer, E / 2003 / Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism by GC-MS / Eur. J. Biochem. 270 : 880 ~ 891
Bailey, J. E / 1991 / Towards a science of metabolic engineering / Science 252 : 1668 ~ 1674
Ben-Israel, A / 1974 / Generalized inverses: Theory and applications / John Wiley & Sons, New York
Christensen, B / 2000 / Metabolic network analysis of P. chrisogenum using 13C-labeled glucose / Biotechnol. Bioeng. 68 : 652 ~ 659
Fischer, E / 2003 / Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism by GC-MS / Eur. J. Biochem. 270 : 880 ~ 891
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