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논문 기본 정보
- 자료유형
- 학술저널
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제7호
- 발행연도
- 2006.1
- 수록면
- 1,032 - 1,040 (9page)
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Some Pseudomonas species can grow on styreneas a sole carbon and energy source. From the new isolatePseudomonas putida SN1, the genes for styrene catabolismwere cloned and sequenced. They were composed of fourstructural genes for styrene monooxygenase (styA and styB),styrene oxide isomerase (styC), and phenylacetaldehydedehydrogenase (styD), along with two genes for the regulatorysystem (styS and styR). All the genes showed high DNAsequence (91% to 99%) and amino acid sequence (94% toreported styrene-degrading Pseudomonas strains. A recombinantEscherichia coli to contain the styrene monooxygenase fromthe SN1 was constructed under the control of the T7 promoterfor the production of enantiopure (S)-styrene oxide, which isan important chiral building block in organic synthesis. Therecombinant E. coli could convert styrene into an enantiopure(S)-styrene oxide (ee >99%) when induced by IPTG. Themaximum activity was observed as 140 U/g cell, when inducedwith 1 mM IPTG at 15oC.
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참고문헌 (23)
참고문헌 신청
Banryx, F / 1999 / Recombinant protein expression in Escherichia coli / Curr. Opin. Biotechnol. 10 : 411 ~ 421
Beltrametti, F / 1997 / Sequencing and functional analysis of styrene catabolism genes from Pseudomonas fluorescens ST. / Appl. Environ. Microbiol. 63 : 2232 ~ 2239
Besse, P / 1994 / Chemical and biological synthesis of chiral epoxides / Tetrahedron 50 : 8885 ~ 8927
Duetz, W. A / 2001 / Using proteins in their natural environment: Potential and limitations of microbial whole-cell hydroxylations in applied biocatalysis / Curr. Opin. Biote
Harayama S / 1992 / Functional and evolutionary relationships among diverse oxygenases / Annu. Rev. Microbiol. 46 : 565 ~ 601
Beltrametti, F / 1997 / Sequencing and functional analysis of styrene catabolism genes from Pseudomonas fluorescens ST. / Appl. Environ. Microbiol. 63 : 2232 ~ 2239
Besse, P / 1994 / Chemical and biological synthesis of chiral epoxides / Tetrahedron 50 : 8885 ~ 8927
Duetz, W. A / 2001 / Using proteins in their natural environment: Potential and limitations of microbial whole-cell hydroxylations in applied biocatalysis / Curr. Opin. Biote
Harayama S / 1992 / Functional and evolutionary relationships among diverse oxygenases / Annu. Rev. Microbiol. 46 : 565 ~ 601
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