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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제6호
- 발행연도
- 2005.1
- 수록면
- 1,304 - 1,309 (6page)
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초록· 키워드
오류제보하기
The production of therapeutic proteins for humandiseases in plants results in many economic benefits, includingreduced risk of animal virus contamination, high yields, andreduced production and storage costs. Human cytokines,interleukin-11 (hIL-11) and granulocyte-macrophage colonystimulatingfactor (hGM-CSF), cDNAs were introduced intorice or tobacco, using either the maize ubiquitin promoter orthe 35S promoter. The primary hIL-11 transgenic rice plantsexhibited stunted growth and a sterile phenotype, whereas thehIL-11 transgenic tobacco plants did not. This suggests thathIL-11 expression in rice disrupts the normal growth anddevelopment of the plant. The regeneration efficiency of ricecalli transformed with hGM-CSF was found to be approximatelya quarter of that seen with the hIL-11, suggesting that hGMCSFexpression is more deleterious to the regeneration of ricecalli than is hIL-11. However, the surviving hGM-CSF transgenicrice plants exhibited a normal phenotype of growth. Therefore,it appears that only those transgenic rice lines that expressedthe human cytokines in small quantities were able to survivethe selection process.
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참고문헌 (24)
참고문헌 신청
/ 1962 / A revised medium forrapid growth and bio assays with tobacco tissue cultures 15 : 473 ~ 497
/ 1980 / Broad host range cloning system for gram negative bacteria Construction of a gene bank of Rhizobium meliloti : 7347 ~ 77 7351
/ 1984 / Transfer of the octopine T-DNA segment to plant cellsmediated by different types of Agrobacterium tumor- orroot-inducing plasmids Generality of virulence systems : 3
/ 1985 / Isolation of cDNA fora human granulocyte-macrophage colony-stimulating factorby functional expression in mammalian cells : 4360 ~ 82 4364
/ 1985 / Molecular cloning of the complementaryDNA and purification of the natural and recombinantproteins : 810 ~ 228 815
/ 1980 / Broad host range cloning system for gram negative bacteria Construction of a gene bank of Rhizobium meliloti : 7347 ~ 77 7351
/ 1984 / Transfer of the octopine T-DNA segment to plant cellsmediated by different types of Agrobacterium tumor- orroot-inducing plasmids Generality of virulence systems : 3
/ 1985 / Isolation of cDNA fora human granulocyte-macrophage colony-stimulating factorby functional expression in mammalian cells : 4360 ~ 82 4364
/ 1985 / Molecular cloning of the complementaryDNA and purification of the natural and recombinantproteins : 810 ~ 228 815
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