지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 105 - 109 (5page)
이용수
초록· 키워드
오류제보하기
Among signal transduction systems by protein
phosphorylation Akt/PKB protein kinase which is one of serine/threonine kinases, is known to regulate the
survival and death of the cell and glucose metabolism. Thus, Akt/PKB protein kinase has been used as one of
the target proteins to find anti-cancer agents from natural products. In this study, human Akt/PKB protein
kinase was expressed in Escherichia coli expression system for the mass production. Human Akt/PKB protein
kinase expressed in E. coli formed inclusion body under the general condition. However, most of the
expressed protein was solubilized under the culture temperature at 27℃ and 0.01-0.09 mM of IPTG for induction
of the protein expression. The expressed protein was purified using Ni2+-NTA agarose column and confirmed
by using anti-Akt antibody. Subsequently, the purified human Akt/PKB protein kinase was activated by
in vitro phosphorylation using cellular extract containing kinases. The activated protein was confirmed to
phosphorylate the specific fluorescent peptide specially designed as the artificial substrate for Akt/PKB protein
kinase.
목차
등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
Bourret, R. B / 1991 / Signal transduction pathways involving protein phosphorylation in prokaryotes / Annu. Rev. Biochem 60 : 401 ~ 441
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Fayard, E / 2005 / Protein kinase B/Akt at a glance / J. Cell Sci 118 : 5675 ~ 5678
Hong, S.-K / 1993 / Effects of protein kinase inhibitors on in vitro protein phosphorylation and cellular differentiation of Streptomyces griseus / Mol. Gen. Genet. 236 : 347
Kang, S. S / 1999 / Akt protein kinase enhances human telomerase activity through phosphorylation of telomerase reverse transcriptase V subunit / J. Biol. Chem 274 : 13085 ~
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Fayard, E / 2005 / Protein kinase B/Akt at a glance / J. Cell Sci 118 : 5675 ~ 5678
Hong, S.-K / 1993 / Effects of protein kinase inhibitors on in vitro protein phosphorylation and cellular differentiation of Streptomyces griseus / Mol. Gen. Genet. 236 : 347
Kang, S. S / 1999 / Akt protein kinase enhances human telomerase activity through phosphorylation of telomerase reverse transcriptase V subunit / J. Biol. Chem 274 : 13085 ~
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
PKB phosphorylates p27, impairs its nuclear import and opposes p27 - mediated G1 arrest
한국생명과학회 심포지움
2002 .09
-
Direct tyrosine phosphorylation of Akt/PKB by epidermal growth factor receptor
생명과학회지
2007 .02
-
광역숙주범위 벡터 pKB1의 조립
미생물학회지
1993 .12
-
Prediction of Protein Kinase Specific Phosphorylation Sites with Multiple SVMs
Bioinformatics and Biosystems
2007 .01
-
Regulation of Skeletal Muscle Differentiation by Akt
생명과학회지
2012 .04
-
Regulation of AKT Activity by Inhibition of the Pleckstrin Homology Domain-PtdIns(3,4,5)P3 Interaction Using Flavonoids
Journal of Microbiology and Biotechnology
2018 .01
-
The pattern of protein phosphorylation by protein kinase C during chondrogenesis in vitro
한국동물학회 학술대회
1996 .01
-
The aspects of protein kinase c isozymes during the induction of apoptosis by protein kinase c inhibitor in PC-3, human prostate cancer cell line
한국동물학회 학술대회
1998 .01
-
Induction of steroid sulfatase expression by tumor necrosis factor-α through phosphatidylinositol 3-kinase/Akt signaling pathway in PC-3 human prostate cancer cells
Experimental and Molecular Medicine
2011 .01
-
Efficient Derivation of New Human Embryonic Stem Cell Lines and Elucidation of the PI3K/Akt/PKB Signal Pathway for the Self Renewal Activity of Human Embryonic Stem Cells
한국동물학회 학술대회
2005 .01
-
Discovery and Evaluation of Polymorphisms in the AKT2 and AKT3 Promoter Regions for Risk of Korean Lung Cancer
Genomics & informatics
2012 .01
-
Role of PI3-Kinase/Akt Pathway in the Activation of Etoposide-Induced NF-κBTranscription Factor
Journal of Microbiology and Biotechnology
2006 .01
-
Translocation of Protein Kinase C Isozymes in the Breast Cancer Cell Line
생명과학회지
1998 .12
-
Development-specific Phosphorylation of the 63-kDa Protein by the 9-type Protein Kinase C in Chick Embryonic Skeletal Muscle
한국동물학회 학술대회
1996 .01
-
Identification of 3'-Hydroxymelanetin and Liquiritigenin as Akt Protein Kinase Inhibitors
Journal of Microbiology and Biotechnology
2006 .01
-
HT-29 대장암세포에서 Akt 활성 저해에 따른 셀레늄의 세포 증식억제 효과
생명과학회지
2012 .01
-
Membrane associated guanylate kinase inverted-3의 AKT signaling을 통한 enterovirus replication 조절
생명과학회지
2016 .10
-
Inhibitory Effect Against Akt of Cyclic Dipeptides Isolated from Bacillus sp
Journal of Microbiology and Biotechnology
2008 .01
-
A GSK-3/SHAGGY-Related Protein Kinase is Involved in Phytochrome Signal Transduction Pathway
Journal of photoscience : an international journal officail organ of the korean society of photoscience
2000 .01
-
고지방식이와 열량제한식이가 백서상피세포의 Protein Kinase C 활성에 미치는 영향
한국영양학회지
1991 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0