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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제9호
- 발행연도
- 2008.1
- 수록면
- 1,529 - 1,536 (8page)
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초록· 키워드
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Acetylation of lysine residues in proteins is a reversible
and highly regulated posttranslational modification. However,
it has not been systematically studied in prokaryotes. By
affinity immunoseparation using an anti-acetyllysine antibody
together with nano-HPLC/MS/MS, we identified 125 lysineacetylated
sites in 85 proteins among proteins derived
from Escherichia coli. The lysine-acetylated proteins identified
are involved in diverse cellular functions including protein
synthesis, carbohydrate metabolism, the TCA cycle, nucleotide
and amino acid metabolism, chaperones, and transcription.
Interestingly, we found a higher level of acetylation during
the stationary phase than in the exponential phase; proteins
acetylated during the stationary phase were immediately
deacetylated when the cells were transferred to fresh LB
culture medium. These results demonstrate that lysine
acetylation is abundant in E. coli and might be involved in
modifying or regulating the activities of various enzymes
involved in critical metabolic processes and the synthesis of
building blocks in response to environmental changes.
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참고문헌 (22)
참고문헌 신청
Barak, R. / 2004 / Acetylation of the chemotaxis response regulator CheY by acetyl-CoA synthetase purified from Escherichia coli / Mol. Biol 342 : 383 ~ 401
Barak, R. / 2006 / The chemotaxis response regulator CheY can catalyze its own acetylation / Mol. Biol 359 : 251 ~ 265
Blander, G. / 2004 / The Sir2 family of protein deacetylases. Annu. Rev / Biochem 73 : 417 ~ 435
Bordone, L. / 2005 / Calorie restriction, SIRT1 and metabolism: Understanding longevity. Nat. Rev. Mol / Cell Biol 6 : 298 ~ 305
Brunet, A. / 2004 / Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase / Science 303 : 2011 ~ 2015
Barak, R. / 2006 / The chemotaxis response regulator CheY can catalyze its own acetylation / Mol. Biol 359 : 251 ~ 265
Blander, G. / 2004 / The Sir2 family of protein deacetylases. Annu. Rev / Biochem 73 : 417 ~ 435
Bordone, L. / 2005 / Calorie restriction, SIRT1 and metabolism: Understanding longevity. Nat. Rev. Mol / Cell Biol 6 : 298 ~ 305
Brunet, A. / 2004 / Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase / Science 303 : 2011 ~ 2015
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