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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 194 - 201 (8page)
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초록· 키워드
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Clitocybin A is a novel anti-wrinkle cosmetic agent produced by the strain from a Korean native mushroom Clitocybe aurantiaca. In this study, fermentation, extraction, and purification conditions for a large scale production of clitocybin A were optimized, and its cytotoxicity and inhibition activity on the expression of matrix metalloproteinase-1 (MMP-1) were characterized. The mass production of anti-wrinkle agent was achieved according to the 300 L fermentation process with a fedbatch cultivation using the modified yeast-maltose (YM) broth, and a total of 12.5 kg of cell mass was obtained in a 120 L culture broth for 14 days. After extraction and purification, clitocybin A was identified by HPLC. The cytotoxicity of clitocybin A was examined by the MTT assay. When assayed at 100 and 200 μg/ml concentrations, clitocybin A showed no cytotoxicity, demonstrating safety. The inhibition activity of clitocybin A on the expression of MMP-1 was examined against UV irradiation. Oleanolic acid (control group) showed a relatively low MMP-1 inhibiting activity (ca. 16.7%) at 10 μg/ml and showed increased cytotoxicity at higher concentrations. In contrast, clitocybin A showed no cytotoxicity at 100 μg/ml, and exhibited a relatively high MMP-1-inhibiting activity (33.1%). These findings indicate that clitocybin A may be a safe and effective anti-wrinkle agent for use in functional cosmetics.
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참고문헌 (21)
참고문헌 신청
Farwick M / 2007 / Salicyloyl-phytosphingosine: a novel agent for the repair of photoaged skin / Int. J. Cosmet. Sci 29 : 319 ~ 329
Han SK / 2013 / Korea national arboretum / Geobook : 386 ~ 393
Kim KB / 2011 / Bioproduction of mushroom mycelium of Agaricus bisporus by commercial submerged fermentation for the production of meat analogue / J. Sci. Food Agric 91 : 156
Kim YH / 2008 / A new oxidant, clitocybin A, from the culture broth of Clitocybe aurantiaca / J. Antibiot 61 : 573 ~ 576
Kim YH / 2008 / Anti-wrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient / Biosci. Biot
Han SK / 2013 / Korea national arboretum / Geobook : 386 ~ 393
Kim KB / 2011 / Bioproduction of mushroom mycelium of Agaricus bisporus by commercial submerged fermentation for the production of meat analogue / J. Sci. Food Agric 91 : 156
Kim YH / 2008 / A new oxidant, clitocybin A, from the culture broth of Clitocybe aurantiaca / J. Antibiot 61 : 573 ~ 576
Kim YH / 2008 / Anti-wrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient / Biosci. Biot
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