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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제2호
- 발행연도
- 2005.1
- 수록면
- 354 - 361 (8page)
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초록· 키워드
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Vibrio fluvialis, an enteropathogenic bacterium,produces a phospholipase which is thought to be an importantfactor in the pathogenesis of disease. In this study, thephospholipase gene (vfp) was identified from V. fluvialis(KCTC 2473) and its sequence was determined. The entireopen reading frame was composed of 1,689 nucleotides and563 amino acids. The phospholipase gene (vfp) was overexpressedin Escherichia coli as a his-tag fused protein. This recombinantprotein (rVFP58) was solubilized with 6 M urea and purifiedby Ni-NTA affinity chromatography. The action mode ofrVFP58 was determined by TLC and GC-MS, and it showedphospholipase B activity, which had both phospholipase Aand lysophospholipase activities. The rVFP58 showed amaximum activity at pH around 9- 10 and temperature ofabout 40oC, and it was stable under alkaline condition overpH 9. The cytotoxicity of rVFP58 was evaluated, using a fishcell line, CHSE-214, and was found to cause significant celldeath after 14 h of exposure to 250 μg of the protein.
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참고문헌 (28)
참고문헌 신청
A / 1995181-188 / Purification and characterization of lysophospholipasetransacylase 1257 (um zawa)
A / 1996 / Evidencefor phospholipase B activity in Fusobacterium necrophorumcultures and its association with hemolysin/leucocidin activities 49 : 219 ~ 233
A / 2000 / PhospholipaseA secreted by Legionella pneumophila destroys alveolarsurfactant phospholipids 188 : 129 ~ 133
A / 2000 / Potential role of phospholipase invirulence and fungal pathogenesis ghan (um) : 122 ~ 143
Alam / 1995 / Production ofantigenically related exocellular elastolytic proteases mediatinghemagglutination by vibrios 39 (l) : 67 ~ 70
A / 1996 / Evidencefor phospholipase B activity in Fusobacterium necrophorumcultures and its association with hemolysin/leucocidin activities 49 : 219 ~ 233
A / 2000 / PhospholipaseA secreted by Legionella pneumophila destroys alveolarsurfactant phospholipids 188 : 129 ~ 133
A / 2000 / Potential role of phospholipase invirulence and fungal pathogenesis ghan (um) : 122 ~ 143
Alam / 1995 / Production ofantigenically related exocellular elastolytic proteases mediatinghemagglutination by vibrios 39 (l) : 67 ~ 70
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Role of Phospholipases in Wound Signal Transduction of Plants
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Isolation and Characterization of Fatty Acid Derivatives from an Actinomycetes and Examination of the Effects on Activities of Phospholipase C and Protein Kinase C
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1998 .12
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Candida albicans가 분비하는 Phospholipase B의 정제 및 특성
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1999 .02
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Decreased Membrane Integrity in Castor Bean Mitochondria by Hydrogen Peroxide. Evidence for the Involvement of Phospholipase D.
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Bioluminescent Assay of Phospholipase C Using A Luminescent Marine Mutant Bacterium Vibrio harveyi M-17
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Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis
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Detection and Identification of Vibrio Species Using Whole-Cell Protein Pattern Analysis
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The p38 mitogen-activated protein kinase is involved in stress-induced phospholipase D activation in vascular smooth muscle cells
Experimental and Molecular Medicine
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