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논문 기본 정보
- 자료유형
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제2호
- 발행연도
- 2008.1
- 수록면
- 228 - 233 (6page)
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Effects of chaperones on mRNA stability and gene expression were studied in order to develop an efficient Escherichia coli expression system that can maximize gene expression. The stability of mRNA was modulated by introducing various secondary structures at the 5'-end of mRNA. Four vector systems providing different 5'-end structures were constructed, and genes encoding GFPuv and endoxylanase were cloned into the four vector systems.
Primer extension assay revealed different mRNA half-lives depending on the 5'-end secondary structures of mRNA.
In addition to the stem-loop structure at the 5'-end of mRNA, coexpression of dnaK-dnaJ-grpE or groEL-groES, representative heat-shock genes in E. coli, increased the mRNA stability and the level of gene expression further, even though the degree of stabilization was varied. Our work suggests that some of the heat-shock proteins can function as mRNA stabilizers as well as protein chaperones.
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참고문헌 (26)
참고문헌 신청
Balbas, P. / 1990 / Design and construction of expression plasmid vectors in Escherichia coli / Methods Enzymol 185 : 14 ~ 37
Bechhofer, D. / 1993 / mRNA stabilizers / Academic Press : 31 ~ 52
Belasco, J. G. / 1986 / The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments / Cell 46 : 245 ~ 251
Bouvet, P. / 1992 / Control of RNase Emediated RNA degradation by 5'-terminal base pairing in E. coli / Nature 360 : 488 ~ 491
Carpousis, A. J. / 2007 / The RNA degradosome of Escherichia coli: A multiprotein mRNA-degrading machine assembled on RNase E. Annu. Rev / Microbiol 61 : 71 ~ 87
Bechhofer, D. / 1993 / mRNA stabilizers / Academic Press : 31 ~ 52
Belasco, J. G. / 1986 / The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments / Cell 46 : 245 ~ 251
Bouvet, P. / 1992 / Control of RNase Emediated RNA degradation by 5'-terminal base pairing in E. coli / Nature 360 : 488 ~ 491
Carpousis, A. J. / 2007 / The RNA degradosome of Escherichia coli: A multiprotein mRNA-degrading machine assembled on RNase E. Annu. Rev / Microbiol 61 : 71 ~ 87
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