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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제1호
- 발행연도
- 2005.1
- 수록면
- 7 - 13 (7page)
이용수
초록· 키워드
오류제보하기
Chitosan-alginate capsules were formed by electrostaticinteractions and exhibited an appropriate mechanical strength,permeability, and stability for the culture of hepatocytes. Pighepatocytes were isolated and hepatocyte spheroids formedand immobilized in chitosan-alginate capsules. An encapsulationprocedure of 3 min and spheroid formation period of 24 hwere the optimum conditions for the best liver functions.Pig hepatocytes with a cell density of 6.0×106 cells/ml inthe capsules were found to be most suitable for applicationin a bioartificial liver support system. The encapsulated pighepatocyte spheroids exhibited stable ammonia removal andurea secretion rates in a bioreactor for 2 weeks. Accordingly,chitosan-alginate encapsulated hepatocyte spheroids in apacked-bed bioreactor would appear to have potential as abioartificial liver.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
A / 1995189-193 / An unlimited resource for bioartificialliver devices 40 : 189 ~ 193
A / 2000 / Encapsulation of hepatocytes by SiO 32 : 2713 ~ 2714
Goosen / 1985 / Optimization of microencapsulation parameters Semipermeable microcapsules as a bioartificial pancreas : 148 ~ 150
Joly / 1997 / and functions of porcine hepatocytes encapsulatedin coated alginate beads A step toward a reliablebioartificial liver 63 : 795 ~ 803
Kim / 1990 / Chitosan for the encapsulationof mammalian cell culture : 617 ~ 626
A / 2000 / Encapsulation of hepatocytes by SiO 32 : 2713 ~ 2714
Goosen / 1985 / Optimization of microencapsulation parameters Semipermeable microcapsules as a bioartificial pancreas : 148 ~ 150
Joly / 1997 / and functions of porcine hepatocytes encapsulatedin coated alginate beads A step toward a reliablebioartificial liver 63 : 795 ~ 803
Kim / 1990 / Chitosan for the encapsulationof mammalian cell culture : 617 ~ 626
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