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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제8호
- 발행연도
- 2006.1
- 수록면
- 1,184 - 1,191 (8page)
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The expression of a pullulanase gene in Pichiawas investigated. The gene encoding pullulanase wascloned by PCR using the chromosomal DNA of Bacilusnaganoensis as the template. The expression vector pPIC9K-Puwas constructed by inserting the pullulanase gene into plasmidpPIC9K and then transformed into Pichia pastoris SMD168by electroporation. Activity determination, SDS-PAGE, andPCR amplification indicated that the gene of the pululanase fromB. naganoensisand the molecular size of the expressed recombinant productwas about 119.9 kDa. This is the first report on the successfulexpression of the pullulanase from B. naganoensis in P.pastoris. The transformant secreted recombinant pululanasewith the activity of 350.8 IU/ml in shake-flask culture. Theproperties of the recombinant pululanase were characterized.
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참고문헌 (16)
참고문헌 신청
Carol, A. S / 1994 / Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression / Biotechnology 12 : 181 ~ 184
Cereghino, J. L / 2000 / Heterologous protein expression in the methylotrophic yeast Pichia pastoris / FEMS Microbiol Rev. 24 : 45 ~ 46
Clare, J / 1998 / Expression of EGF and HIV envelope glycoprotein / Methods Mol. Biol. 103 : 209 ~ 225
Clare, J. J / 1991 / High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integration of the gene / Biotechnology 9 : 455 ~
Cregg, J. M / 1993 / Recent advances in the expression of foreign genes in Pichia pastoris / Biotechnology 11 : 905 ~ 910
Cereghino, J. L / 2000 / Heterologous protein expression in the methylotrophic yeast Pichia pastoris / FEMS Microbiol Rev. 24 : 45 ~ 46
Clare, J / 1998 / Expression of EGF and HIV envelope glycoprotein / Methods Mol. Biol. 103 : 209 ~ 225
Clare, J. J / 1991 / High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integration of the gene / Biotechnology 9 : 455 ~
Cregg, J. M / 1993 / Recent advances in the expression of foreign genes in Pichia pastoris / Biotechnology 11 : 905 ~ 910
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Pullulanase 를 생산하는 Bacillus 속 세균의 분리와 효소의 최적 생산조건 및 특성 ( Isolation of Bacillus sp. Producing Pullulanase and Culture Conditions for Production and Properties of the Enzyme )
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1996 .06
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항균활성을 보유한 재조합 Pichia pastoris 균주의 개발
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High-Level Expression of an Aspergillus niger Endo-β-1,4-Glucanase in Pichia pastoris Through Gene Codon Optimization and Synthesis
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Methylotrophic Yeast, Pichia pastoris에서 사람 락토페린의 발현 및 항균성 연구
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Cloning and Overexpression of a Paenibacillus β-Glucanase in Pichia pastoris:Purification and Characterization of the Recombinant Enzyme
Journal of Microbiology and Biotechnology
2007 .01
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Purification and Biochemical Properties of an Alkaline Pullulanase from Alkalophilic Bacillus sp. S-1
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1998 .01
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Expression and Characterization of the Human rpS3 in a Methylotrophic Yeast Pichia pastoris
The Journal of Microbiology
2000 .06
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Bacillus sp. S-1으로부터 생성된 새로운 균체의 pullulanase의 생화학적 특성 ( Biochemical characterization of a novel extracellular pullulan 6-glucanohydrolase from Bacillus circulans S-1 )
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2000 .06
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A Possible Mechanism Responsible for Translocation and Secretion of an Alkaliphilic Bacillus sp. S-1 Pullulanase
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Expression of Human Lactoferrin N-lobe in Pichia pastoris and Its Antibacterial Activity
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Cloning, High-Level Expression, Purification, and Properties of a Novel Endo-β-1,4-Mannanase from Bacillus subtilis G1 in Pichia pastoris
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Pichia pastoris: A Recombinant Microfactory for Antibodies and Human Membrane Proteins
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Deletion Analysis of Pichia PGK1 Promoter and Construction of anEpisomal Vector for Heterologous Protein Expression in P. pastoris
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A Specific Pullulanase for α-1,6-Glucosidic Linkage of Glucan from Thermus caldophilus
Journal of Life Science
1999 .08
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Optimization of the Functional Expression of Coprinus cinereus Peroxidase in Pichia pastoris by Varying the Host and Promoter
Journal of Microbiology and Biotechnology
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Transformation of Terpene Synthase from Polyporus brumalis in Pichia pastoris for Recombinant Enzyme Production
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A New Signal Sequence for Recombinant Protein Secretion in Pichia pastoris
Journal of Microbiology and Biotechnology
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