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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제9호
- 발행연도
- 2010.1
- 수록면
- 1,359 - 1,366 (8page)
이용수
초록· 키워드
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A search of the Streptomyces avermitilis genome reveals that its closest homologs are several O-methyltransferases.
Among them, one gene (viz., saomt5) was cloned into the pET-15b expression vector by polymerase chain reaction using sequence-specific oligonucleotide primers. Biochemical characterization with the recombinant protein showed that SaOMT5 was S-adenosyl-L-methionine-dependent Omethyltransferase.
Several compounds were tested as substrates of SaOMT5. As a result, SaOMT5 catalyzed Omethylation of flavonoids such as 6,7-dihydroxyflavone,2',3'-dihydroxyflavone, 3',4'-dihydroxyflavone, quercetin,and 7,8-dihydroxyflavone, and phenolic compounds such as caffeic acid and caffeoyl Co-A. These reaction products were analyzed by TLC, HPLC, LC/MS, and NMR spectroscopy. In addition, SaOMT5 could convert phenolic compounds containing ortho-dihydroxy groups into Omethylated compounds, and 6,7-dihydroxyflavone was known to be the best substrate. SaOMT5 converted 6,7-dihydroxyflavone into 6-hydroxy-7-methoxyflavone and 7-hydroxy-6-methoxyflavone, and caffeic acid into ferulic acid and isoferulic acid, respectively. Moreover, SaOMT5turned out to be a Mg2+-dependent OMT, and the effect of Mg2+ ion on its activity was five times greater than those of Ca2+, Fe2+, and Cu2+ ions, EDTA, and metal-free medium.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Bentley, S. D / 2002 / Complete genome sequence of the model actinomycete Streptomyces coelicolorA3(2) / Nature 417 : 141 ~ 147
Dhar, K / 2000 / Purification and characterization of Streptomyces griseus catechol O-methyltransferase / Appl. Environ. Microbiol 66 : 4877 ~ 4882
Dixon, R. A / 1999 / Activation structure and organization of genes involved in microbial defense in plants / Adv. Genet 28 : 165 ~ 234
Dixon, R. A / 2001 / Natural products and plant disease resistance / Nature 411 : 843 ~ 847
Ferrer, J. L / 2005 / Crystal structures of alfalfa caffeoyl coenzyme A 3-Omethyltransferase / Plant Physiol 137 : 1009 ~ 1017
Dhar, K / 2000 / Purification and characterization of Streptomyces griseus catechol O-methyltransferase / Appl. Environ. Microbiol 66 : 4877 ~ 4882
Dixon, R. A / 1999 / Activation structure and organization of genes involved in microbial defense in plants / Adv. Genet 28 : 165 ~ 234
Dixon, R. A / 2001 / Natural products and plant disease resistance / Nature 411 : 843 ~ 847
Ferrer, J. L / 2005 / Crystal structures of alfalfa caffeoyl coenzyme A 3-Omethyltransferase / Plant Physiol 137 : 1009 ~ 1017
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