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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제5호
- 발행연도
- 2016.1
- 수록면
- 928 - 937 (10page)
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S-Nitrosoglutathione reductase (GSNOR) metabolizes S-nitrosoglutathione (GSNO) and has been shown to play important roles in regulating cellular signaling and formulating host defense by modulating intracellular nitric oxide levels. The enzyme has been found in bacterial, yeast, mushroom, plant, and mammalian cells. However, to date, there is still no evidence of its occurrence in filamentous fungi. In this study, we cloned and investigated a GSNOR-like enzyme from the filamentous fungus Aspergillus nidulans. The enzyme occurred in native form as a homodimer and exhibited low thermal stability. GSNO was an ideal substrate for the enzyme. The apparent Km and kcat values were 0.55 mM and 34,100 min-1, respectively. Substrate binding sites and catalytic center amino acid residues based on those from known GSNORs were conserved in this enzyme, and the corresponding roles were verified using site-directed mutagenesis. Therefore, we demonstrated the presence of GSNOR in a filamentous fungus for the first time.
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참고문헌 (40)
참고문헌 신청
Arasimowicz-Jelonek M / 2014 / Nitric oxide: an effective weapon of the plant or the pathogen / Mol. Plant Pathol 15:406~416
Attarian R / 2009 / Glutathione disulfide and S-nitrosoglutathione detoxification by Mycobacterium tuberculosis thioredoxin system / FEBS Lett 583:3215~3220
Bateman RL / 2008 / Human carbonyl reductase 1 is an S-nitrosoglutathione reductase / J. Biol. Chem 283:35756~35762
Benhar M / 2008 / Regulated protein denitrosylation by cytosolic and mitochondrial thioredoxins / Science 320:1050~1054
Benhar M / 2009 / Protein denitrosylation: enzymatic mechanisms and cellular functions / Nat. Rev. Mol. Cell Biol 10:721~732
Attarian R / 2009 / Glutathione disulfide and S-nitrosoglutathione detoxification by Mycobacterium tuberculosis thioredoxin system / FEBS Lett 583:3215~3220
Bateman RL / 2008 / Human carbonyl reductase 1 is an S-nitrosoglutathione reductase / J. Biol. Chem 283:35756~35762
Benhar M / 2008 / Regulated protein denitrosylation by cytosolic and mitochondrial thioredoxins / Science 320:1050~1054
Benhar M / 2009 / Protein denitrosylation: enzymatic mechanisms and cellular functions / Nat. Rev. Mol. Cell Biol 10:721~732
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