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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 288 - 299 (12page)
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초록· 키워드
오류제보하기
Food bio preservation is of major interest in the food industry. Many types of antimicrobial compounds can be produced by lactic acid bacteria (LAB), including bacteriocins. Bacteriocins increase the shelf-life of food by decreasing some food-borne diseases. In this study, a multi-coding sequence of bacteriocin genes was used for primer design to produce bacteriocin genes in Enterococcus faecium AH2 strain and Pediococcus pentosaceus AH1. Multi-coding sequences were aligned to detect conserved sequences in the bacteriocin gene. Eight genes encoding proteins involved in bacteriocin production were isolated and sequenced, including six from E. faecium AH2 (entA, entI, entF, entR, orfA2, orfA3) and two from P. pentoceseus AH1 (papA, pedB), and all gene sequences were deposited in the Gen Bank database under accession numbers LC064146–LC064151, LC101300, and LC101789, respectively. P. pentosaceus AH1 and E. faecium AH2 strains displayed bacteriocin activities of 2610 AU mL-1and 690 AU mL-1and inhibition zones of 26 mm and 19 mm, respectively. Overexpression of entA in E. faecium AH2 increased the bacteriocin and antimicrobial activities.
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등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Choffnes ER / 2012 / Improving food safety through a one health approach: workshop summary / National Academies Press
Morovsky M / 2001 / Isolation and characterization of enterocin BC25 and occurrence of the entA gene among ruminal Gram-positive cocci / Microbiol. Res. 156:133~138
Qi F / 1999 / Purification of mutacin III from group III Streptococcus mutans UA787 and genetic analyses of mutacin III biosynthesis genes / Appl. Environ. Microbiol. 65:3880~3887
O'Keeffe T / 1999 / Characterization and heterologous expression of the genes encoding enterocin A production, immunity, and regulation in Enterococcus faecium DPC1146 / Appl. Environ. Microbiol. 65:1506~1515
Franz CM / 1999 / Atypical genetic locus associated with consti- tutive production of enterocin B by Enterococcus faecium BFE 900 / Appl. Environ. Microbiol. 65:2170~2178
Morovsky M / 2001 / Isolation and characterization of enterocin BC25 and occurrence of the entA gene among ruminal Gram-positive cocci / Microbiol. Res. 156:133~138
Qi F / 1999 / Purification of mutacin III from group III Streptococcus mutans UA787 and genetic analyses of mutacin III biosynthesis genes / Appl. Environ. Microbiol. 65:3880~3887
O'Keeffe T / 1999 / Characterization and heterologous expression of the genes encoding enterocin A production, immunity, and regulation in Enterococcus faecium DPC1146 / Appl. Environ. Microbiol. 65:1506~1515
Franz CM / 1999 / Atypical genetic locus associated with consti- tutive production of enterocin B by Enterococcus faecium BFE 900 / Appl. Environ. Microbiol. 65:2170~2178
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