지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제4호
- 발행연도
- 2012.1
- 수록면
- 530 - 534 (5page)
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초록· 키워드
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A real-time PCR assay was developed and validated inhouse specifically for the detection of Clostridium perfringens (Cl. perfringens) in meats and vegetables by comparing with the culture method. The detection limit of the real-time PCR assay in phosphate-buffered saline was 102 CFU/ml.
When the two methods were compared in food samples inoculated with Cl. perfringens, the culture method detected 52 positives, whereas real-time PCR detected 51positives out of 160 samples. The difference was without statistical significance (p>0.05). Real-time PCR assay is an option for quality assurance laboratories to perform standard diagnostic tests, considering its detection ability and time-saving efficiency.
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참고문헌 (16)
참고문헌 신청
Albini, S. / 2008 / Real-time multiplex PCR assays for reliable detection of Clostridium perfringens toxin genes in animal isolates / Vet. Microbiol 127 : 179 ~ 185
Brynestad, S. / 2002 / Clostridium perfringens and food borne infections / Int. J. Food Microbiol 74 : 195 ~ 202
Cai, T. / 2006 / Application of real-time PCR for quantitative detection of Vibrio parahaemolyticus from seafood in eastern China / FEMS Immunol. Med. Microbiol 46 : 180 ~ 18
Erol, I. / 2008 / Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR / Lett. Appl. Microbiol 47 : 31 ~ 34
Fukushima, H. / 2007 / Rapid separation and concentration of food-borne pathogensin food samples prior to quantification by viable-cell countingand real-time PCR / Appl. Envi
Brynestad, S. / 2002 / Clostridium perfringens and food borne infections / Int. J. Food Microbiol 74 : 195 ~ 202
Cai, T. / 2006 / Application of real-time PCR for quantitative detection of Vibrio parahaemolyticus from seafood in eastern China / FEMS Immunol. Med. Microbiol 46 : 180 ~ 18
Erol, I. / 2008 / Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR / Lett. Appl. Microbiol 47 : 31 ~ 34
Fukushima, H. / 2007 / Rapid separation and concentration of food-borne pathogensin food samples prior to quantification by viable-cell countingand real-time PCR / Appl. Envi
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