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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제4호
- 발행연도
- 2012.1
- 수록면
- 510 - 515 (6page)
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초록· 키워드
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A recombinant putative dextransucrase (DexT) was produced from Leuconostoc citreum KM20 as a 160 kDa protein, but its productivity was very low (264 U/l). For optimization,we examined enzyme activity in 7 Escherichia coli strains with inducer molecules such as lactose or IPTG. E. coli BL21-CodonPlus(DE3)-RIL exhibited the highest enzyme activity with lactose. Finally, DexT activity was remarkably increased by 12-fold under the optimized culture conditions of a cell density to start induction (OD600) of 0.95, a lactose concentration of 7.5 mM, and an induction temperature of 17oC. These results may effectively apply to the heterologous expression of other large DexT genes.
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참고문헌 (19)
참고문헌 신청
Ashipala, O. K. / 2008 / Optimization of fibrinolytic enzyme production by Bacillus subtilis DC-2 in aqueous twophase system (poly-ethylene glycol 4000 and sodium sulfate) /
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
Britton, H. T. S. / 1931 / Universal buffer solutions and dissociation constant of veronal / J. Chem. Soc : 1456 ~ 1462
Choi, I. K. / 2003 / Novel Leuconostoc citreum starter culture system for the fermentation of kimchi, a fermented cabbage product / Antonie Van Leeuwenhoek 84 : 247 ~ 253
Henrissat, B. / 1997 / Structural and sequence based classification of glycoside hydrolases / Curr. Opin. Struct. Biol 7 : 637 ~ 644
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
Britton, H. T. S. / 1931 / Universal buffer solutions and dissociation constant of veronal / J. Chem. Soc : 1456 ~ 1462
Choi, I. K. / 2003 / Novel Leuconostoc citreum starter culture system for the fermentation of kimchi, a fermented cabbage product / Antonie Van Leeuwenhoek 84 : 247 ~ 253
Henrissat, B. / 1997 / Structural and sequence based classification of glycoside hydrolases / Curr. Opin. Struct. Biol 7 : 637 ~ 644
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