지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 303 - 309 (7page)
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Keratinases are of particular interest because of their action on insoluble keratins and generally on a broad range of protein substrates. Alkalophilic and neutrophilic actinomycete strains isolated from different soil samples, rich in keratinaceous substances were screened for keratinolytic activity. An alkalophilic isolate,TBG-S13A5, was found to possess good keratinolytic activity and was able to utilize feather as the sole carbon and nitrogen source. TBG-S13A5 exhibited an off-white aerial mass color with a rectus-flexibilis type of spore chain. The morphological, microscopical and biochemical characters were comparable with that of Streptomyces albidoflavus. Fatty acid methyl ester profiling (FAME) and 16S rDNA sequence analysis confirmed its identity as a strain of S. albidoflavus. Under submerged fermentation conditions, maximum protease production was recorded on the 5th day of incubation at 30oC, using basal broth of pH 9.0 with 0.25% (w/v)white chicken feather. This strain could affect feather degradation when the initial pH was 8 and above and maximum protease production was recorded when the initial pH was around 10.5. The effectiveness of the crude enzyme in destaining and leather dehairing were also demonstrated.
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참고문헌 (25)
참고문헌 신청
Agrahari, S / 2010 / Degradation of chicken feather a poultry waste product by keratinolytic bacteria iso lated from dumping site at Ghazipur poultry processing plant / Int.
Anonymous / / Interpreting Sherlock Reports. p. 4-1-4-9. In: MIS Operating Manual, Version 6.0, Sherlock® Microbial Identification System
Bascaran, V / 1990 / Regulation of extracellular protease production in Streptomyces clavuligerus / Appl. Microbiol. Biotechnol 34 : 208 ~ 213
Bressollier, P / 1999 / Purification and characterization of a keratinolytic serine proteinase from Streptomyces albidoflavus / Appl. Environ. Microbiol 65 : 2570 ~ 2576
Cai, C. / 2009 / Medium optimization for keratinase production in hair substrate by a new Bacillus subtilis KD-N2 using response surface methodology / J. Ind. Microbiol. Biot
Anonymous / / Interpreting Sherlock Reports. p. 4-1-4-9. In: MIS Operating Manual, Version 6.0, Sherlock® Microbial Identification System
Bascaran, V / 1990 / Regulation of extracellular protease production in Streptomyces clavuligerus / Appl. Microbiol. Biotechnol 34 : 208 ~ 213
Bressollier, P / 1999 / Purification and characterization of a keratinolytic serine proteinase from Streptomyces albidoflavus / Appl. Environ. Microbiol 65 : 2570 ~ 2576
Cai, C. / 2009 / Medium optimization for keratinase production in hair substrate by a new Bacillus subtilis KD-N2 using response surface methodology / J. Ind. Microbiol. Biot
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