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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제6호
- 발행연도
- 2011.1
- 수록면
- 627 - 636 (10page)
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초록· 키워드
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A commercially important alkaline protease, produced by Bacillus sp. RRM1 isolated from the red seaweed Kappaphycus alvarezii (Doty) Doty ex Silva, was first recognized and characterized in the present study. Identification of the isolated bacterium was done using both biochemical characterization as well as 16S rRNA gene sequencing. The bacterial strain, Bacillus sp. RRM1, produced a high level of protease using easily available, inexpensive agricultural residues solid-state fermentation (SSF). Among them,wheat bran was found to be the best substrate. Influences of process parameters such as moistening agents, moisture level, temperature, inoculum concentration, and co-carbon and co-nitrogen sources on the fermentation were also evaluated. Under optimized conditions, maximum protease production (i.e., 2081 U/g) was obtained from wheat bran,which is about 2-fold greater than the initial conditions.
The protease enzyme was stable over a temperature range of 30-60℃ and pH 6-12, with maximum activity at 50℃and pH 9.0. Whereas the metal ions Na+, Ca²+, and K+enhanced the activity of the enzyme, others such as Hg²+,Cu²+, Fe²+, Co²+, and Zn²+ had rendered negative effects.
The activity of the enzyme was inhibited by EDTA and enhanced by Cu²+ ions, thus indicating the nature of the enzyme as a metalloprotease. The enzyme showed extreme stability and activity even in the presence of detergents,surfactants, and organic solvents. Moreover, the present findings opened new vistas in the utilization of wheat bran, a cheap, abundantly available, and effective waste as a substrate for SSF.
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참고문헌 (23)
참고문헌 신청
Adreas, K. G. / 1999 / Lipase production by Penicillium restricum in solid state fermentation using Babassu oil cake as substrate / Process Biochem 35 : 85 ~ 90
Aikat, K / 2000 / Protease extraction in solid-state fermentation of wheat bran by a local strain of Rhizopus oryzae and growth studies by the soft gel technique / Process Bi
Annapurna, R. A / 1996 / Eco-friendly enzymatic dehairing using extracellular protease from Bacillus species isolate / J. Am. Leath. Chem. Assoc 91 : 115 ~ 119
Battan, B / 2006 / High level xylanase production by alkaliphlic Bacillus pumilus ASH under solid state fermentation / World J. Microbiol. Biotechnol 22 : 1281 ~ 1287
Beg, Q. K / 2002 / De-repression and subsequent induction of protease synthesis by Bacillus mojavensis under fed-batch operations / Process Biochem 37 : 1103 ~ 1109
Aikat, K / 2000 / Protease extraction in solid-state fermentation of wheat bran by a local strain of Rhizopus oryzae and growth studies by the soft gel technique / Process Bi
Annapurna, R. A / 1996 / Eco-friendly enzymatic dehairing using extracellular protease from Bacillus species isolate / J. Am. Leath. Chem. Assoc 91 : 115 ~ 119
Battan, B / 2006 / High level xylanase production by alkaliphlic Bacillus pumilus ASH under solid state fermentation / World J. Microbiol. Biotechnol 22 : 1281 ~ 1287
Beg, Q. K / 2002 / De-repression and subsequent induction of protease synthesis by Bacillus mojavensis under fed-batch operations / Process Biochem 37 : 1103 ~ 1109
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