지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제11호
- 발행연도
- 2013.1
- 수록면
- 1,627 - 1,635 (9page)
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Mitochondria often play central roles in apoptotic pathways, and disruption of the mitochondrial transmembrane potential (ΔΨm) has been observed in various cells undergoing apoptosis. Human cytomegalovirus (HCMV) infection induces apoptosis in permissive cells;however, investigations of mitochondria-targeted apoptosis in HCMV-infected human foreskin fibroblast (HFF) cells have been limited. Here, we investigated the mitochondrial apoptosis pathway in HCMV-infected HFF cells. Flow cytometry analysis using JC-1 revealed that HCMV infection induces disruption of ΔΨm in HFF cells when administered 24 h postinfection (hpi), and this disruption was maximized at 48 hpi. Moreover, cytochrome c,normally a mitochondrial inner membrane protein, was detected in cytoplasmic extracts of HCMV-infected cells, but not mock-infected cells, by western blot analysis at 24 hpi. A caspase activity assay based on fluorescence spectrophotometry using a fluorogenic substrate revealed an increase in caspase-3 activity at 48 hpi in HCMV-infected cells. Caspase-8 activity was increased at 72 hpi in HCMV-infected cells. These results imply that HCMV infection induces mitochondria-mediated apoptosis in HFF cells.
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참고문헌 (27)
참고문헌 신청
Afford S / 2000 / Apoptosis / Mol. Pathol 53 : 55 ~ 63
Andoniou CE / 2006 / Insights into the mechanism of CMV-mediated interference with cellular apoptosis / Immunol. Cell Biol 84 : 99 ~ 106
Armstrong JS / 2006 / The role of the mitochondrial permeability transition in cell death / Mitochondrion 6 : 225 ~ 234
Arnoult D / 2004 / Cytomegalovirus cell death suppressor vMIA blocks Bax- but not Bak-mediated apoptosis by binding and sequestering Bax at mitochondria / Proc. Natl. Acad. S
Bedner E / 1999 / Analysis of apoptosis by laser scanning cytometry / Cytometry 35 : 181 ~ 195
Andoniou CE / 2006 / Insights into the mechanism of CMV-mediated interference with cellular apoptosis / Immunol. Cell Biol 84 : 99 ~ 106
Armstrong JS / 2006 / The role of the mitochondrial permeability transition in cell death / Mitochondrion 6 : 225 ~ 234
Arnoult D / 2004 / Cytomegalovirus cell death suppressor vMIA blocks Bax- but not Bak-mediated apoptosis by binding and sequestering Bax at mitochondria / Proc. Natl. Acad. S
Bedner E / 1999 / Analysis of apoptosis by laser scanning cytometry / Cytometry 35 : 181 ~ 195
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