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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제3호
- 발행연도
- 2016.1
- 수록면
- 588 - 595 (8page)
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초록· 키워드
오류제보하기
IFN-γ release assays (IGRAs) have been developed as viable alternative diagnostic tools for detecting latent tuberculosis infection (LTBI). A customized homogeneous sandwich luminescent oxygen channeling immunoassay (LOCI) was used to quantify IFN-γ levels in IGRAs. Samples were collected from healthy volunteers (n = 40) who were T-Spot-negative and T-Spot-positive patients (n = 32) at rest. Then the amount of IFN-γ in the supernatant of IGRAs was measured by LOCI. The results demonstrated a low background, and high sensitivity, specificity, accuracy, and reproducibility, and a short assay time (only 30 min) with LOCI for IFN-γ. The recovery range was 81.63-102.06%, the coefficients of variation were below 5%, and the limit of detection was 19.0 mIU/ml. Excellent agreement between LOCI IFN-γ and the T-SPOT.TB test was obtained (97.2% agreement, κ = 0.94). The LOCI IFN-γ concentrations were significantly higher in T-Spot-positive patients than in the healthy group (p < 0.001). Moreover, as observed for the comparative LOCI IFN-γ assay, IFN-γ concentrations were related to the numbers of T-SPOT.TB spots. We have established an in vitro blood test for LTBI diagnosis, defined as LOCI IFN-γ. A high level of agreement between the LOCI IFN-γ method and T-SPOT.TB assay was observed in clinical studies that showed the LOCI IFN-γ method could determine LTBI. This study shows acceptable performance characteristics of the LOCI IFN-γ assay to diagnose LTBI.
목차
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참고문헌 (23)
참고문헌 신청
Barry CE / 2009 / The spectrum of latent tuberculosis: rethinking the biology and intervention strategies / Nat. Rev. Microbiol 7:845~855
Beaudet L / 2001 / Homogeneous assays for single-nucleotide polymorphism typing using AlphaScreen / Gen. Res 11:600~608
Bosse R / 2000 / Miniaturizing screening: how low can we go today / Drug Discov. Today :42~47
Cohn DL / 2000 / Targeted tuberculin testing and treatment of latent tuberculosis infection / MMWR Morb. Mortal. Wkly. Rep 49:1~54
Connell TG / 2006 / Performance of a whole blood interferon gamma assay for detecting latent infection with Mycobacterium tuberculosis in children / Thorax 61:616~620
Beaudet L / 2001 / Homogeneous assays for single-nucleotide polymorphism typing using AlphaScreen / Gen. Res 11:600~608
Bosse R / 2000 / Miniaturizing screening: how low can we go today / Drug Discov. Today :42~47
Cohn DL / 2000 / Targeted tuberculin testing and treatment of latent tuberculosis infection / MMWR Morb. Mortal. Wkly. Rep 49:1~54
Connell TG / 2006 / Performance of a whole blood interferon gamma assay for detecting latent infection with Mycobacterium tuberculosis in children / Thorax 61:616~620
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