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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제11호
- 발행연도
- 2016.1
- 수록면
- 1,924 - 1,932 (9page)
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초록· 키워드
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Mycophenolic acid (MPA) is an antibiotic produced by Penicillium brevicompactum. MPA has antifungal, antineoplastic, and immunosuppressive functions, among others. β-Hydroxy-β- methylglutaryl-CoA (HMG-CoA) lyase is a key enzyme in the bypass metabolic pathway. The inhibitory activity of HMG-CoA lyase increases the MPA biosynthetic flux by reducing the generation of by-products. In this study, we cloned the P. brevicompactum HMG-CoA lyase gene using the thermal asymmetric interlaced polymerase chain reaction and gene walking technology. Agrobacterium tumefaciens-mediated transformation (ATMT) was used to insert a mutated HMG-CoA lyase gene into P. brevicompactum. Successful insertion of the HMG-CoA lyase gene was confirmed by hygromycin screening, PCR, Southern blot analysis, and enzyme content assay. The maximum MPA production by transformants was 2.94 g/l. This was 71% higher than wild-type ATCC 16024. Our results demonstrate that ATMT may be an alternative practical genetic tool for directional transformation of P. brevicompactum.
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참고문헌 (25)
참고문헌 신청
Allison AC / 2000 / Immunosuppressive drugs: the first 50years and a glance forward / Immunopharmacology 47 : 63 ~ 83
An G / 1988 / Plant Molecular Biology Manual / Kluwer Academic Publishers : 1 ~ 19
Ando K / 1968 / Antiviral activity of mycophenolic acid. Studies on antiviral and antitumor antibiotics / J. Antibiot 21 : 649 ~ 652
Be dford CT / 1973 / Biosynthesis of mycophenolic acid / Can. J. Chem 51 : 694 ~ 697
Bentley R / 2000 / Mycophenolic acid: a one hundred year odyssey from antibiotic to immunosuppressant / Chem. Rev 100 : 3801 ~ 3826
An G / 1988 / Plant Molecular Biology Manual / Kluwer Academic Publishers : 1 ~ 19
Ando K / 1968 / Antiviral activity of mycophenolic acid. Studies on antiviral and antitumor antibiotics / J. Antibiot 21 : 649 ~ 652
Be dford CT / 1973 / Biosynthesis of mycophenolic acid / Can. J. Chem 51 : 694 ~ 697
Bentley R / 2000 / Mycophenolic acid: a one hundred year odyssey from antibiotic to immunosuppressant / Chem. Rev 100 : 3801 ~ 3826
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