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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제11호
- 발행연도
- 2006.1
- 수록면
- 1,799 - 1,808 (10page)
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초록· 키워드
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A food-grade integration vector based on site-specific recombination was constructed. The 5.7-kb vector,pIMA20, contained an integrase gene and a phage attachmentsite originating from bacteriophage A2, with the α-galactosidasegene from Lactobacillus plantarum KCTC 3104 as a selectionpromoter of nisA (PnisA) and a signal peptide-encodingsequence of usp45 (SPusp45) for the production and secretionof foreign proteins. pIMA20 and its derivatives mediated site-specific integration into the attB-like site on the Lactococcuslactis NZ9800 chromosome. The vector-integrated recombinantlactocci were easily detected by the apearance of bluecolonies on a medium containing X-α-gal and also by theirability to grow on a medium containing melibiose as the solecarbon source. Recombinant lactococci maintained these traitsin the absence of selection pressure during 100 generations.The α-amylase gene from Bacilus licheniformis, lacking asignal peptide-encoding sequence, was inserted downstreamof PnisA and SPusp45 in pIMA20, and the plasmid was integratedinto the L. lactis chromosome. α-Amylase was successfullyproduced and secreted by the recombinant L. lactis, controlledby the adition and concentration of nisin.
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참고문헌 (22)
참고문헌 신청
Alvarez, M. A / 1998 / The sitespecific recombination system of the Lactobacillus species bacteriophage A2 integrates in Gram-positive and Gramnegative bacteria / Virology 25
Atlung, T / 1991 / A versatile method for integration of genes and gene fusions into the λ attachment site of Escherichia coli / Gene 107 : 11 ~ 17
Bermudez-Humaran, L. G / 2003 / Controlled intra- or extracellular production of staphylococcal nuclease and ovine omega interferon in Lactococcus lactis / FEMS Microbiol. Le
Brondsted, L. / 1999 / Use of the integration elements encoded by the temperate lactococcal bacteriophage TP901-1 to obtain chromosomal single-copy transcriptional fusions in
CHOI, HAK-JONG / 2005 / Heterologous Expression of Human β-Defensin-1 in Bacteriocin-Producing Lactococcus lactis / Journal of Microbiology and Biotechnology 15 (2) : 330 ~
Atlung, T / 1991 / A versatile method for integration of genes and gene fusions into the λ attachment site of Escherichia coli / Gene 107 : 11 ~ 17
Bermudez-Humaran, L. G / 2003 / Controlled intra- or extracellular production of staphylococcal nuclease and ovine omega interferon in Lactococcus lactis / FEMS Microbiol. Le
Brondsted, L. / 1999 / Use of the integration elements encoded by the temperate lactococcal bacteriophage TP901-1 to obtain chromosomal single-copy transcriptional fusions in
CHOI, HAK-JONG / 2005 / Heterologous Expression of Human β-Defensin-1 in Bacteriocin-Producing Lactococcus lactis / Journal of Microbiology and Biotechnology 15 (2) : 330 ~
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